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Western Blot (WB) (Western Blot detection against Immunogen (75.17kD).)

Mouse anti-Human, Mouse NADK Monoclonal Antibody | anti-NADK antibody

NADK (NAD Kinase, Poly(P)/ATP NAD Kinase, dJ283E3.1, DKFZp686L22239, FLJ13052, FLJ37724, FLJ54695, FLJ77769, FLJ78247, FLJ78307, MGC1900)

Gene Names
NADK; dJ283E3.1; RP1-283E3.6
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
NADK; Monoclonal Antibody; NADK (NAD Kinase; Poly(P)/ATP NAD Kinase; dJ283E3.1; DKFZp686L22239; FLJ13052; FLJ37724; FLJ54695; FLJ77769; FLJ78247; FLJ78307; MGC1900); Anti -NADK (NAD Kinase; anti-NADK antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
5F4
Specificity
Recognizes human NADK. Species Crossreactivity: mouse.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MEMEQEKMTMNKELSPDAAAYCCSACHGDETWSYNHPIRGRAKSRSLSASPALGSTKEFRRTRSLHGPCPVTTFGPKACVLQNPQTIMHIQDPASQRLTWNKSPKSVLVIKKMRDASLLQPFKELCTHLMEENMIVYVEKKVLEDPAIASDESFGAVKKKFCTFREDYDDISNQIDFIICLGGDGTLLYASSLFQGSVPPVMAFHLGSLGFLTPFSFENFQSQVTQVIEGNAAVVLRSRLKVRVVKELRGKKTAVHNGLGEKGSQAAGLDMDVGKQAMQYQVLNEVVIDRGPSSYLSNVDVYLDGHLITTVQGDGVIVSTPTGSTAYAAAAGASMIHPNVPAIMITPICPHSLSFRPIVVPAGVELKIMLSPEARNTAWVSFDGRKRQEIRHGDSISITTSCYPLPSICVRDPVSDWFESLAQCLHWNVRKKQAHFEEEEEEEEEG
Applicable Applications for anti-NADK antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Immunogen
Full length recombinant corresponding to aa1-447 from NADK (AAH01709) with GST tag. MW of the GST tag alone is 26kD.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (75.17kD).)

Western Blot (WB) (Western Blot detection against Immunogen (75.17kD).)

Western Blot (WB)

(NADK monoclonal antibody. Western Blot analysis of NADK expression in Raw 264.7.)

Western Blot (WB) (NADK monoclonal antibody. Western Blot analysis of NADK expression in Raw 264.7.)

Western Blot (WB)

(NADK monoclonal antibody. Western Blot analysis of NADK expression in NIH/3T3.)

Western Blot (WB) (NADK monoclonal antibody. Western Blot analysis of NADK expression in NIH/3T3.)

Western Blot (WB)

(Western Blot analysis of NADK expression in transfected 293T cell line by NADK monoclonal antibody.|Lane 1: NADK transfected lysate (49.2kD).|Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of NADK expression in transfected 293T cell line by NADK monoclonal antibody.|Lane 1: NADK transfected lysate (49.2kD).|Lane 2: Non-transfected lysate.)

Testing Data

(Detection limit for recombinant GST tagged NADK is ~0.3ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged NADK is ~0.3ng/ml as a capture antibody.)

Western Blot (WB)

(NADK monoclonal antibody, Western Blot analysis of NADK expression in HeLa.)

Western Blot (WB) (NADK monoclonal antibody, Western Blot analysis of NADK expression in HeLa.)

Western Blot (WB)

(NADK monoclonal antibody. Western Blot analysis of NADK expression in HepG2.)

Western Blot (WB) (NADK monoclonal antibody. Western Blot analysis of NADK expression in HepG2.)
Product Categories/Family for anti-NADK antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,228 Da
NCBI Official Full Name
NAD kinase isoform 3
NCBI Official Synonym Full Names
NAD kinase
NCBI Official Symbol
NADK
NCBI Official Synonym Symbols
dJ283E3.1; RP1-283E3.6
NCBI Protein Information
NAD kinase; poly(P)/ATP NAD kinase
UniProt Protein Name
NAD kinase
Protein Family
UniProt Gene Name
NADK
UniProt Entry Name
NADK_HUMAN

NCBI Description

NADK catalyzes the transfer of a phosphate group from ATP to NAD to generate NADP, which in its reduced form acts as an electron donor for biosynthetic reactions (Lerner et al., 2001 [PubMed 11594753]).[supplied by OMIM, Mar 2008]

Uniprot Description

NADK: Belongs to the NAD kinase family

Protein type: Kinase, other; EC 2.7.1.23; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: cytosol

Molecular Function: metal ion binding; ATP binding; NAD+ kinase activity

Biological Process: ATP metabolic process; vitamin metabolic process; NAD metabolic process; NADP biosynthetic process; phosphorylation; water-soluble vitamin metabolic process

Research Articles on NADK

Similar Products

Product Notes

The NADK nadk (Catalog #AAA6009783) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NADK (NAD Kinase, Poly(P)/ATP NAD Kinase, dJ283E3.1, DKFZp686L22239, FLJ13052, FLJ37724, FLJ54695, FLJ77769, FLJ78247, FLJ78307, MGC1900) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NADK can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Researchers should empirically determine the suitability of the NADK nadk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MEMEQEKMTM NKELSPDAAA YCCSACHGDE TWSYNHPIRG RAKSRSLSAS PALGSTKEFR RTRSLHGPCP VTTFGPKACV LQNPQTIMHI QDPASQRLTW NKSPKSVLVI KKMRDASLLQ PFKELCTHLM EENMIVYVEK KVLEDPAIAS DESFGAVKKK FCTFREDYDD ISNQIDFIIC LGGDGTLLYA SSLFQGSVPP VMAFHLGSLG FLTPFSFENF QSQVTQVIEG NAAVVLRSRL KVRVVKELRG KKTAVHNGLG EKGSQAAGLD MDVGKQAMQY QVLNEVVIDR GPSSYLSNVD VYLDGHLITT VQGDGVIVST PTGSTAYAAA AGASMIHPNV PAIMITPICP HSLSFRPIVV PAGVELKIML SPEARNTAWV SFDGRKRQEI RHGDSISITT SCYPLPSICV RDPVSDWFES LAQCLHWNVR KKQAHFEEEE EEEEEG. It is sometimes possible for the material contained within the vial of "NADK, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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