IL-1 alpha /IL-1F1 Active Protein | IL-1F1 active protein

Recombinant IL-1 alpha /IL-1F1

Cat. Num. AAA201129

• Gene Names: IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA; IL-1 alpha
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: IL-1 alpha /IL-1F1; Recombinant IL-1 alpha /IL-1F1; Interleukin-1 alpha; IL-1 alpha; Hematopoietin-1; Interleukin-1 alpha: Interleukin-1a; IL-1a; IL1A; interleukin 1 alpha; IL1F1; IL-1A; preinterleukin 1 alpha; pro-interleukin-1-alpha; IL-1F1 active protein

• Host: E Coli
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid; 20mM Tris-HCl pH 7.5, 10% Glycerol.
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 113-271aa
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHM SA PFSFLSNVKY NFMRIIKYEF ILNDALNQSI IRANDQYLTA AALHNLDEAV KFDMGAYKSS KDDAKITVIL RISKTQLYVT AQDEDQPVLL KEMPEIPKTI TGSETNLLFF WETHGTKNYF TSVAHPNLFI ATKQDYWVCL AGGPPSITDF QILENQA
• Sequence Length: 271

• Applicable Applications for IL-1F1 active protein: SDS-PAGE, Bioactivity
• Species: Human
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Biological Activity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 for this effect is less or equal to 0.04 ng/ml.
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Related Product Information for IL-1F1 active protein

IL-1 consists of two distinct forms, IL-1 alpha and IL-1 beta which perform the same functions but are distinct proteins. IL-1alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. Recombinant human IL-1alpha was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for IL-1F1 active protein

Hormones & Cytokines

References

Misener VL., et al. (2008). Neuropsychobiology. 58(2):71-80. Sarkar O., et al. (2008). Biol Reprod. 78(3):445-54.

NCBI and Uniprot Product Information

• NCBI GI #: 27894330
• NCBI GeneID: 3552
• NCBI Accession #: NP_000566.3
• NCBI GenBank Nucleotide #: NP_000566.3
• UniProt Accession #: P01583
• Molecular Weight: 22.4kDa (197aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: interleukin-1 alpha
• NCBI Official Synonym Full Names: interleukin 1 alpha
• NCBI Official Symbol: IL1A
• NCBI Official Synonym Symbols: IL1; IL-1A; IL1F1; IL1-ALPHA; IL-1 alpha
• NCBI Protein Information: interleukin-1 alpha
• UniProt Protein Name: Interleukin-1 alpha
• UniProt Gene Name: IL1A
• UniProt Synonym Gene Names: IL1F1; IL-1 alpha
• UniProt Entry Name: IL1A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]

Uniprot Description

IL1A: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Belongs to the IL-1 family.

Protein type: Motility/polarity/chemotaxis; Cytokine

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: extracellular space; extracellular region; cytosol

Molecular Function: protein binding; interleukin-1 receptor binding; copper ion binding; cytokine activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of mitosis; apoptosis; positive regulation of interleukin-2 biosynthetic process; cytokine and chemokine mediated signaling pathway; germ cell programmed cell death; positive regulation of JNK cascade; fever; negative regulation of cell proliferation; cell proliferation; connective tissue replacement during inflammatory response; positive regulation of angiogenesis; response to copper ion; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response; positive regulation of cytokine secretion