SDS-Page
sodA active protein
sodA, 1-206aa, E Coli, His tag, E Coli (Bioactivity Validated)
Gene Names
sodA; ECK3901; JW3879
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
sodA; 1-206aa; E Coli; His tag; E Coli (Bioactivity Validated); Superoxide dismutase manganese; Superoxide dismutase; manganese; MnSOD; sodA active protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MSYTLPSLPY AYDALEPHFD KQTMEIHHTK HHQTYVNNAN AALESLPEFA NLPVEELITK LDQLPADKKT VLRNNAGGHA NHSLFWKGLK KGTTLQGDLK AAIERDFGSV DNFKAEFEKA AASRFGSGWA WLVLKGDKLA VVSTANQDSP LMGEAISGAS GFPIMGLDVW EHAYYLKFQN RRPDYIKEFW NVVNWDEAAA RFAAKK
Sequence Length
206
Applicable Applications for sodA active protein
SDS-PAGE
Antigen Species
E Coli
Tag Information
His-tag
Biological Activity
Specific activity is > 300 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.
Assay
1. Prepare a 180ul reaction mix into a suitable container and pre-chill on ice before use: The final concentrations are 48.6mM potassium phosphate, 5mM ethylendiaminetetraacetic acid, 60mM cytochromC, 0.78mM xanthine, 0.01 units xanthine oxidase.
2. Equilibrate to 25C and monitor at A550nm until the value is constant using a spectrophotometer.
3. Add 20ul of recombinant sodA protein protein to 50ug/ml in assay buffer.
4. Mix by inversion and record the increase at A550nm for 5 minutes.
2. Equilibrate to 25C and monitor at A550nm until the value is constant using a spectrophotometer.
3. Add 20ul of recombinant sodA protein protein to 50ug/ml in assay buffer.
4. Mix by inversion and record the increase at A550nm for 5 minutes.
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for sodA active protein
Superoxide dismutase, Mn, also known as sodA, is a member of the iron/manganese superoxide dismutase family. SodA destroys radicals which are normally produced within the cells and which are toxic to biological systems. It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase. Recombinant E Coli sodA protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for sodA active protein
References
Barrister J V., et al. (1987) Crit Rev Biochem. 22:11-180.; ujika I., et al. (1991) Acta Histochem Cytochem. 24:489-495.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25.2 kDa (226aa) confirmed by MALDI-TOF
NCBI Official Full Name
superoxide dismutase, Mn
NCBI Official Symbol
sodA
NCBI Official Synonym Symbols
ECK3901; JW3879
NCBI Protein Information
superoxide dismutase, Mn
UniProt Protein Name
Superoxide dismutase [Mn]
Protein Family
UniProt Gene Name
sodA
NCBI Description
SoxRS regulon. [More information is available at EcoGene: EG10953].
Uniprot Description
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Research Articles on sodA
Similar Products
Product Notes
The sodA soda (Catalog #AAA204096) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's sodA can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the sodA soda for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MSYTLPSLPY AYDALEPHFD KQTMEIHHTK HHQTYVNNAN AALESLPEFA NLPVEELITK LDQLPADKKT VLRNNAGGHA NHSLFWKGLK KGTTLQGDLK AAIERDFGSV DNFKAEFEKA AASRFGSGWA WLVLKGDKLA VVSTANQDSP LMGEAISGAS GFPIMGLDVW EHAYYLKFQN RRPDYIKEFW NVVNWDEAAA RFAAKK. It is sometimes possible for the material contained within the vial of "sodA, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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