SDS-Page
SOD2 active protein
SOD2, 25-222aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
SOD2; IPOB; IPO-B; MNSOD; MVCD6; Mn-SOD
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
SOD2; 25-222aa; Human; His tag; E Coli (Bioactivity Validated); Superoxide dismutase [Mn] mitochondrial; IPOB; MNSOD; MVCD6; Superoxide dismutase [Mn]; mitochondrial IPO B; Manganese SOD; Manganese superoxide dismutase; Mn SOD; SOD 2; Superoxide dismutase 2 mitochondrial; Superoxide Dismutase-2; SOD2 active protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MKHSLPDLPY DYGALEPHIN AQIMQLHHSK HHAAYVNNLN VTEEKYQEAL AKGDVTAQIA LQPALKFNGG GHINHSIFWT NLSPNGGGEP KGELLEAIKR DFGSFDKFKE KLTAASVGVQ GSGWGWLGFN KERGHLQIAA CPNQDPLQGT TGLIPLLGID VWEHAYYLQY KNVRPDYLKA IWNVINWENV TERYMACKK
Sequence Length
222
Applicable Applications for SOD2 active protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Biological Activity
Specific activity is > 1,000 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.
Assay
1. Prepare a 180ul assay buffer into a suitable container and pre-chill on ice before use: The concentrations are 54mM potassium phosphate, 5.5mM ethylendiaminetetraacetic acid, 66mM cytochromC 0.9mM xanthine, 0.01 units xanthine oxidase.
2. Equilibrate to 25C and monitor at A550nm until the value is constant using a spectrophotometer.
3. Add 20ul of recombinant SOD2 protein to 50ug/ml in assay buffer.
4. Mix by inversion and record the increase at A550nm for 5 minutes.
2. Equilibrate to 25C and monitor at A550nm until the value is constant using a spectrophotometer.
3. Add 20ul of recombinant SOD2 protein to 50ug/ml in assay buffer.
4. Mix by inversion and record the increase at A550nm for 5 minutes.
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for SOD2 active protein
SOD2 is a member of the iron/manganese superoxide dismutase family. It is a mitochondrial matrix protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this protein have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Recombinant human SOD2 protein was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for SOD2 active protein
References
MacMillan-Crow L.A., et al. (1999) Arch. Biochem. Biophys. 366:82-88; Prunotto M, et al. (2010) J Am Soc Nephrol. 21 (3):507-19.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24.4kDa (219aa), confirmed by MALDI-TOF.
NCBI Official Full Name
superoxide dismutase
NCBI Official Synonym Full Names
superoxide dismutase 2
NCBI Official Symbol
SOD2
NCBI Official Synonym Symbols
IPOB; IPO-B; MNSOD; MVCD6; Mn-SOD
NCBI Protein Information
superoxide dismutase [Mn], mitochondrial
UniProt Protein Name
Superoxide dismutase [Mn], mitochondrial
Protein Family
UniProt Gene Name
SOD2
NCBI Description
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1. [provided by RefSeq, Apr 2016]
Uniprot Description
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Research Articles on SOD2
Similar Products
Product Notes
The SOD2 sod2 (Catalog #AAA203695) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's SOD2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the SOD2 sod2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MKHSLPDLPY DYGALEPHIN AQIMQLHHSK HHAAYVNNLN VTEEKYQEAL AKGDVTAQIA LQPALKFNGG GHINHSIFWT NLSPNGGGEP KGELLEAIKR DFGSFDKFKE KLTAASVGVQ GSGWGWLGFN KERGHLQIAA CPNQDPLQGT TGLIPLLGID VWEHAYYLQY KNVRPDYLKA IWNVINWENV TERYMACKK. It is sometimes possible for the material contained within the vial of "SOD2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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