ALDH2 active protein

ALDH2,18-517aa, Human, E Coli (Bioactivity Validated)

Cat. Num. AAA203287

• Gene Names: ALDH2; ALDM; ALDHI; ALDH-E2
• Reactivity: Human
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: ALDH2; 18-517aa; Human; E Coli (Bioactivity Validated); Aldehyde dehydrogenase mitochondrial; ALDH-E2; ALDHI; ALDM; Aldehyde dehydrogenase; mitochondrial Acetaldehyde dehydrogenase 2; Aldehyde dehydrogenase 2 family; Aldehyde dehydrogenase 2 family (mitochondrial); ALDH 2; ALDH class 2; ALDH E2; Liver mitochondrial ALDH; MGC1806; Mitochondrial aldehyde dehydrogenase 2; Nucleus encoded mitochondrial aldehyde dehydrogenase 2.; ALDH2 active protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 7.5) containing 1mM DTT, 1 mM EDTA, 10% glycerol
• Concentration: 1 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S
• Sequence Length: 517

• Applicable Applications for ALDH2 active protein: SDS-PAGE; Enzyme Activity
• Domain: 18-517aa
• Endotoxin Level: < 1.0 EU per 1 ug of protein (determined by LAL method)
• Biological Activity: Specific activity is > 250pmol/min/ug, and was obtained by measuring the increase of NADH in absorbance at 340 nm resulting from the reduction of NAD at pH 8.0 at 25°C.
• Preparation and Storage: Can be stored at +2°C to +8°C for one week.; For long term storage, aliquot and store at -20 degree C or -80 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Related Product Information for ALDH2 active protein

ALDH2 (Aldehyde dehydrogenase 2 family) belongs to the aldehyde dehydrogenase family which catalyze the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. There are two major liver isoforms of this enzyme, cytosolic and mitochondrial, and they can be also distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Recombinant human ALDH2 protein was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for ALDH2 active protein

Redox Proteins

References

Crabb DW., et al. (1989). J Clin Invest. 83 (1):314-6; Feng Liu., et al. (2002). Plant Physiol. 130 (4):1657-1674;

NCBI and Uniprot Product Information

• NCBI GI #: 25777732
• NCBI GeneID: 217
• NCBI Accession #: NP_000681.2
• NCBI GenBank Nucleotide #: NM_000690.3
• UniProt Accession #: P05091; Q03639; Q6IB13; Q6IV71; B4DW54; E7EUE5
• Molecular Weight: 54.5 kDa (501aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: aldehyde dehydrogenase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 2 family member
• NCBI Official Symbol: ALDH2
• NCBI Official Synonym Symbols: ALDM; ALDHI; ALDH-E2
• NCBI Protein Information: aldehyde dehydrogenase, mitochondrial
• UniProt Protein Name: Aldehyde dehydrogenase, mitochondrial
• UniProt Gene Name: ALDH2
• UniProt Synonym Gene Names: ALDM

NCBI Description

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of East Asians have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among East Asians than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Nov 2016]