GSTA4 active protein

GSTA4, 1-222aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA206246

• Gene Names: GSTA4; GTA4; GSTA4-4
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: GSTA4; 1-222aa; Human; His tag; E Coli (Bioactivity Validated); Glutathione S-transferase alpha 4; GSTA4-4; GTA4; GSTA4 active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0), 2mM DTT, 20% glycerol, 100mM NaCl
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAARPK LHYPNGRGRM ESVRWVLAAA GVEFDEEFLE TKEQLYKLQD GNHLLFQQVP MVEIDGMKLV QTRSILHYIA DKHNLFGKNL KERTLIDMYV EGTLDLLELL IMHPFLKPDD QQKEVVNMAQ KAIIRYFPVF EKILRGHGQS FLVGNQLSLA DVILLQTILA LEEKIPNILS AFPFLQEYTV KLSNIPTIKR FLEPGSKKKP PPDEIYVRTV YNIFRP
• Sequence Length: 222

• Applicable Applications for GSTA4 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity is > 4,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
• Assay: 1. Prepare a 180ul assay buffer into a suitable container. The concentrations are 106mM potassium phosphate (pH6.5), 1.1mM EDTA, 5.5mM glutathione, reduced, 2.2mM 1-chloro-2,4-dinitrobenzene (CDNB), 8% (v/v) ethanol.; 2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer.; 3. Add 20ul of recombinant GSTA4 protein 40ug/ml in assay buffer.; 4. Mix by inversion and record the decrease at A340nm for approximately 5 minutes.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GSTA4 active protein

GSTA4, also known as glutathione S-transferase A4, belongs to the GST superfamily. This enzyme is involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. GSTA4 shows very high activity with reactive carbonyl compounds such as alk-2-enals. GSTA4 is highly effective in catalyzing the conjugate addition of reduced glutathione to 4-hydroxynonenal, an important product of peroxidative degradation of arachidonic acid and a commonly used biomarker for oxidative damage in tissue. This enzyme is expressed at a high level in brain, placenta, and skeletal muscle and much lower in lung and liver. Recombinant human GSTA4 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for GSTA4 active protein

Metabolism

References

Bruns C.M., et al. (1999) J. Mol. Biol. 288:427-439; Balogh L.M., et al. (2010) Biochemistry 49:1541-1548.

NCBI and Uniprot Product Information

• NCBI GI #: 4504173
• NCBI GeneID: 2941
• NCBI Accession #: NP_001503
• NCBI GenBank Nucleotide #: NM_001512.3
• UniProt Accession #: O15217; Q5T7Q8; Q6P4G1; Q9BX18; Q9H414; B2RD15
• Molecular Weight: 28.3kDa (246aa) confirmed by MALDI-TOF
• NCBI Official Full Name: glutathione S-transferase A4
• NCBI Official Synonym Full Names: glutathione S-transferase alpha 4
• NCBI Official Symbol: GSTA4
• NCBI Official Synonym Symbols: GTA4; GSTA4-4
• NCBI Protein Information: glutathione S-transferase A4
• UniProt Protein Name: Glutathione S-transferase A4
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTA4

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, which are located in a cluster on chromosome 6, are highly related and encode enzymes with glutathione peroxidase activity that function in the detoxification of lipid peroxidation products. Reactive electrophiles produced by oxidative metabolism have been linked to a number of degenerative diseases including Parkinson's disease, Alzheimer's disease, cataract formation, and atherosclerosis. [provided by RefSeq, Jul 2008]

Uniprot Description

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This isozyme has a high catalytic efficiency with 4-hydroxyalkenals such as 4-hydroxynonenal (4-HNE).