GSTM3 active protein

GSTM3, 1-225aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA204379

• Gene Names: GSTM3; GST5; GSTB; GTM3; GSTM3-3
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: GSTM3; 1-225aa; Human; His tag; E Coli (Bioactivity Validated); Glutathione S-transferase mu 3; GST5; GSTB; GSTM3-3; GTM3; GSTM3 active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSCESS MVLGYWDIRG LAHAIRLLLE FTDTSYEEKR YTCGEAPDYD RSQWLDVKFK LDLDFPNLPY LLDGKNKITQ SNAILRYIAR KHNMCGETEE EKIRVDIIEN QVMDFRTQLI RLCYSSDHEK LKPQYLEELP GQLKQFSMFL GKFSWFAGEK LTFVDFLTYD ILDQNRIFDP KCLDEFPNLK AFMCRFEALE KIAAYLQSDQ FCKMPINNKM AQWGNKPVC
• Sequence Length: 225

• Applicable Applications for GSTM3 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity is > 15,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
• Assay: 1. Prepare a 180ul assay buffer into a suitable container. The concentrations are 106mM potassium phosphate (pH6.5), 1.1mM EDTA, 5.5mM glutathione, reduced, 2.2mM 1-chloro-2,4-dinitrobenzene (CDNB), 8% (v/v) ethanol.; 2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer.; 3. Add 20ul of recombinant GSTM3 protein 10ug/ml in assay buffer.; 4. Mix by inversion and record the decrease at A340nm for approximately 5 minutes.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GSTM3 active protein

Glutathione S-transferase mu 3, also known as GSTM3, is member of the glutathione s-transferase (GST) family of proteins. There are eight families of GST proteins, namely alpha, kappa, mu, omega, pi, sigma, theta and zeta, each of which is composed of proteins that have a variety of functions throughout the cell. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTM3 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for GSTM3 active protein

Metabolism

References

Boqaards JJ., et al. (1992) Biochem J. 289 (2):383-8.; Rozell B., et al. (1992) Xenobiotica. 23 (8):835-49.

NCBI and Uniprot Product Information

• NCBI GI #: 23065552
• NCBI GeneID: 2947
• NCBI Accession #: NP_000840
• NCBI GenBank Nucleotide #: NM_000849.4
• UniProt Accession #: P21266; O60550; Q96HA3
• Molecular Weight: 29.1 kDa (249aa), confirmed by MALDI-TOF
• NCBI Official Full Name: glutathione S-transferase Mu 3
• NCBI Official Synonym Full Names: glutathione S-transferase mu 3
• NCBI Official Symbol: GSTM3
• NCBI Official Synonym Symbols: GST5; GSTB; GTM3; GSTM3-3
• NCBI Protein Information: glutathione S-transferase Mu 3
• UniProt Protein Name: Glutathione S-transferase Mu 3
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTM3
• UniProt Synonym Gene Names: GST5; hGSTM3-3

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Mutations of this class mu gene have been linked with a slight increase in a number of cancers, likely due to exposure with environmental toxins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]

Uniprot Description

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. May govern uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers.