SDS-Page
SOD1 active protein
SOD1, 1-154aa, Human, E Coli (Bioactivity Validated)
Gene Names
SOD1; ALS; SOD; ALS1; IPOA; hSod1; HEL-S-44; homodimer
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
SOD1; 1-154aa; Human; E Coli (Bioactivity Validated); Superoxide dismutase 1 soluble; ALS; SOD; ALS1; IPOA; Cu-Zn superoxide dismutase; Superoxide dismutase 1; soluble ALS 1; Amyotrophic lateral sclerosis 1 Amyotrophic lateral sclerosis 1 adult; Cu/Zn SOD; Cu/Zn superoxide dismutase; Homodimer; Indophenoloxidase A; Mn superoxide dismutase; SOD 1; SOD soluble; SOD2; SODC; Superoxide dismutase Cu Zn; Superoxide dismutase cystolic; ; SOD1 active protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid in 20 mM Tris pH 7.5, 10% glycerol.
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MATKAVCVLK GDGPVQGIIN FEQKESNGPV KVWGSIKGLT EGLHGFHVHE FGDNTAGCTS AGPHFNPLSR KHGGPKDEER HVGDLGNVTA DKDGVADVSI EDSVISLSGD HCIIGRTLVV HEKADDLGKG GNEESTKTGN AGSRLACGVI GIAQ
Sequence Length
154
Applicable Applications for SOD1 active protein
SDS-PAGE
Antigen Species
Human
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Biological Activity
Specific activity is > 1,000 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.
Assay
1. Prepare a 180ul assay buffer mix into a suitable container and pre-chill on ice before use: The concentrations are 54mM potassium phosphate, 5.5mM ethylendiaminetetraacetic acid, 66mM
cytochromC 0.9mM xanthine, 0.01 units xanthine oxidase. 2. Equilibrate to 25C and monitor at A550nm until the value is constant using a spectrophotometer.
3. Add 20ul of recombinant SOD1 protein to 50ug/ml in assay buffer.
4. Mix by inversion and record the increase at A550nm for 5 minutes.
cytochromC 0.9mM xanthine, 0.01 units xanthine oxidase. 2. Equilibrate to 25C and monitor at A550nm until the value is constant using a spectrophotometer.
3. Add 20ul of recombinant SOD1 protein to 50ug/ml in assay buffer.
4. Mix by inversion and record the increase at A550nm for 5 minutes.
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for SOD1 active protein
Superoxide dismutase 1 (SOD1) binds copper and zinc ions and is one of three isozymes responsible for destroying free superoxide radicals in the body. The encoded protein neutralizes supercharged oxygen molecules, which can damage cells if their levels are not controlled. Mutations in SOD1 cause a form of familial amyotrophic lateral sclerosis (ALS). Recombinant SOD1 was expressed in E Coli and purified by conventional chromatography techniques.
Product Categories/Family for SOD1 active protein
References
Conwit R., et al. (2006) Journal of the Neurological Sciences 251 (1-2).; Banci L., et al. (2008) PLoS ONE. 3 (2):e1677.
; This product was used in the following article:; Kwon MJ et.al Superoxide dismutase 3 suppresses hyaluronic http://www.ncbi.nlm.nih.gov/pubmed/21957979;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15.9 kDa (154 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
superoxide dismutase
NCBI Official Synonym Full Names
superoxide dismutase 1
NCBI Official Symbol
SOD1
NCBI Official Synonym Symbols
ALS; SOD; ALS1; IPOA; hSod1; HEL-S-44; homodimer
NCBI Protein Information
superoxide dismutase [Cu-Zn]
UniProt Protein Name
Superoxide dismutase [Cu-Zn]
Protein Family
UniProt Gene Name
SOD1
UniProt Synonym Gene Names
hSod1
NCBI Description
The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
Research Articles on SOD1
Similar Products
Product Notes
The SOD1 sod1 (Catalog #AAA203282) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SOD1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the SOD1 sod1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MATKAVCVLK GDGPVQGIIN FEQKESNGPV KVWGSIKGLT EGLHGFHVHE FGDNTAGCTS AGPHFNPLSR KHGGPKDEER HVGDLGNVTA DKDGVADVSI EDSVISLSGD HCIIGRTLVV HEKADDLGKG GNEESTKTGN AGSRLACGVI GIAQ. It is sometimes possible for the material contained within the vial of "SOD1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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