Western Blot (WB)
((1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat TBK1 Polyclonal Antibody | anti-TBK1 antibody
Goat anti-TBK1 (aa514-527) Antibody
Western Blot: Approx 85kDa band observed in lysates of cell line HeLa (calculated MW of 83.6kDa according to NP_037386.1). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 24kDa. This band was successfully blocked by incubation with the immunizing peptide.
Western Blot (WB)
((1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. [provided by RefSeq, Oct 2010]
Uniprot Description
TBK1: a protein kinase of the IKK family. Can mediate NFkB activation in response to certain growth factors. Forms a complex with the IKB protein TANK and TRAF2 and release the NFKB inhibition caused by TANK.
Protein type: EC 2.7.11.1; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; IKK family
Chromosomal Location of Human Ortholog: 12q14.1
Cellular Component: cytoplasm; cytosol; endosome membrane
Molecular Function: ATP binding; nucleic acid binding; phosphoprotein binding; protein binding; protein kinase activity; protein serine/threonine kinase activity
Biological Process: defense response to Gram-positive bacterium; defense response to virus; I-kappaB kinase/NF-kappaB cascade; inflammatory response; innate immune response; interferon type I production; negative regulation of interferon type I production; peptidyl-serine phosphorylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of interferon-alpha production; positive regulation of interferon-beta biosynthetic process; positive regulation of interferon-beta production; positive regulation of peptidyl-serine phosphorylation; positive regulation of transcription from RNA polymerase II promoter; response to virus; viral reproduction
Disease: Frontotemporal Dementia And/or Amyotrophic Lateral Sclerosis 4
Research Articles on TBK1
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Product Notes
The TBK1 tbk1 (Catalog #AAA423480) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TBK1 (aa514-527) Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's TBK1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 85kDa band observed in lysates of cell line HeLa (calculated MW of 83.6kDa according to NP_037386.1). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 24kDa. This band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the TBK1 tbk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TIETSLQDID SRLS. It is sometimes possible for the material contained within the vial of "TBK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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