Rabbit anti-Human NAT1 Polyclonal Antibody | anti-NAT1 antibody

NAT1, ID (NAT1, AAC1, Arylamine N-acetyltransferase 1, Arylamide acetylase 1, Monomorphic arylamine N-acetyltransferase, N-acetyltransferase type 1) (APC)

Cat. Num. AAA6323881

• Gene Names: NAT1; AAC1; MNAT; NATI; NAT-1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NAT1; Polyclonal Antibody; ID (NAT1; AAC1; Arylamine N-acetyltransferase 1; Arylamide acetylase 1; Monomorphic arylamine N-acetyltransferase; N-acetyltransferase type 1) (APC); N-acetyltransferase type 1; anti-NAT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 290

• Applicable Applications for anti-NAT1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-130 amino acids from the Central region of human NAT1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NAT1 Antibody (center) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the NAT1 antibody detected the NAT1 protein (arrow).)

Related Product Information for anti-NAT1 antibody

This gene is one of two arylamine N-acetyltransferase (NAT) genes in the human geneome, and is orthologous to the mouse and rat Nat2 genes. The enzyme encoded by this gene catalyzes the transfer of an acetyl group from acetyl-CoA to various arylamine and hydrazine substrates. This enzyme helps metabolize drugs and other xenobiotics, and functions in folate catabolism. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-NAT1 antibody

Antibodies; Enzymes; Acetyltransferase

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010): Minchin, R.F., et al. Int. J. Biochem. Cell Biol. 39(11):1999-2005(2007). Barker, D.F., et al. Pharmacogenet. Genomics 16(7):515-525(2006). Boukouvala, S., et al. Basic Clin. Pharmacol. Toxicol. 96(5):343-351(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 237649126
• NCBI GeneID: 9
• NCBI Accession #: NP_001153645.1; NP_001153642.1; NP_000653.3; NP_001153643.1; NP_001153644.1
• NCBI GenBank Nucleotide #: NP_001153645.1
• UniProt Accession #: P18440
• NCBI Official Full Name: arylamine N-acetyltransferase 1 isoform a
• NCBI Official Synonym Full Names: N-acetyltransferase 1
• NCBI Official Symbol: NAT1
• NCBI Official Synonym Symbols: AAC1; MNAT; NATI; NAT-1
• NCBI Protein Information: arylamine N-acetyltransferase 1
• UniProt Protein Name: Arylamine N-acetyltransferase 1
• Protein Family: Nucleobase-ascorbate transporter
• UniProt Gene Name: NAT1
• UniProt Synonym Gene Names: AAC1; MNAT; NAT-1
• UniProt Entry Name: ARY1_HUMAN

NCBI Description

This gene is one of two arylamine N-acetyltransferase (NAT) genes in the human genome, and is orthologous to the mouse and rat Nat2 genes. The enzyme encoded by this gene catalyzes the transfer of an acetyl group from acetyl-CoA to various arylamine and hydrazine substrates. This enzyme helps metabolize drugs and other xenobiotics, and functions in folate catabolism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

NAT1: Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates and is able to bioactivate several known carcinogens. Belongs to the arylamine N-acetyltransferase family.

Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Acetyltransferase; Secondary Metabolites Metabolism - caffeine; EC 2.3.1.5

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: cytosol

Molecular Function: arylamine N-acetyltransferase activity

Biological Process: xenobiotic metabolic process