Activity
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DGKH recombinant protein
DGKH, Active
Synonyms
DGKH; Active; Diacylglycerol kinase eta; Diglyceride kinase eta; DGKH recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT and 25% glycerol.
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot targets into smaller quantities after centrifugation and store at the recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see the related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see the related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for DGKH recombinant protein
Recombinant full-length human DGKH was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_178009.5.
DGKH is a type II DGK encoded by gene DGKH (1). Diacylglycerol kinases modulate the concentration of intracellular diacylglycerol by phosphorylating, producing phosphatidic acid (PA)(2). PA regulates signaling proteins, such as protein kinases and phosphatases, and can induce cell mitosis through regulation of growth factors (3). DGKH is a positive regulator of the epidermal growth factor receptor/Raf/MEK/ERK pathway. DGKH is associated with attention deficit hyperactivity disorder, unipolar depression and schizophrenia (4).
DGKH is a type II DGK encoded by gene DGKH (1). Diacylglycerol kinases modulate the concentration of intracellular diacylglycerol by phosphorylating, producing phosphatidic acid (PA)(2). PA regulates signaling proteins, such as protein kinases and phosphatases, and can induce cell mitosis through regulation of growth factors (3). DGKH is a positive regulator of the epidermal growth factor receptor/Raf/MEK/ERK pathway. DGKH is associated with attention deficit hyperactivity disorder, unipolar depression and schizophrenia (4).
Product Categories/Family for DGKH recombinant protein
References
1. Rittiner, JE. et al: Overexpression of diacylglycerol kinase eta enhances Galphaq-coupled G protein-coupled receptor signaling. Mol Pharmacol. 2014; 85(5):800-810.
2. Ding, L. et al: The cloning and characterization of a novel human diacylglycerol kinase, DGKiota. J Biol Chem. 1998; 273(49):32746-52.
3. Buckland, AG. et al: Anionic phospholipids, interfacial binding and the regulation of cell functions. Biochim Biophys Acta. 2000; 1483(2):199-216.
4. Sakane, F. et al: Diacylglycerol kinases as emerging potential drug targets for a variety of diseases: An update. Front Cell Dev Biol. 2016; 4:82.
2. Ding, L. et al: The cloning and characterization of a novel human diacylglycerol kinase, DGKiota. J Biol Chem. 1998; 273(49):32746-52.
3. Buckland, AG. et al: Anionic phospholipids, interfacial binding and the regulation of cell functions. Biochim Biophys Acta. 2000; 1483(2):199-216.
4. Sakane, F. et al: Diacylglycerol kinases as emerging potential drug targets for a variety of diseases: An update. Front Cell Dev Biol. 2016; 4:82.
NCBI and Uniprot Product Information
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Product Notes
The DGKH (Catalog #AAA516802) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "DGKH, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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