DGKK recombinant protein

DGKK Active

Cat. Num. AAA516807

• Synonyms: DGKK; DGKK Active; Diacylglycerol kinase kappa; DGK-kappa; DGKK recombinant protein

• Host: Sf9 insect cells
• Form/Format: Recombinant protein stored in 50mM sodium phosphate, pH7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

• Tag: HIS
• Species: Human
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Activity

(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet)

SDS-Page

(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)

Related Product Information for DGKK recombinant protein

Recombinant full-length human DGKK was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. The gene accession number is NP_001013764.1.
Diacylglycerol kinase kappa (DGKK) is a member of the type II diacylglycerol kinase subfamily. Diacylglycerol kinases modulate the concentration of intracellular diacylglycerol by phosphorylating, producing phosphatidic acid (PA)(1). PA regulates signaling proteins, such as protein kinases and phosphatases, and can induce cell mitosis through regulation of growth factors (2). Genetic variants of DGKk are associated with hypospadias in mild and moderate Han Chinese cases (3).

Product Categories/Family for DGKK recombinant protein

Lipid Kinases; Angiogenesis; Apoptosis/Autophagy; Cancer; Cardiovascular Disease; Cell Cycle; Lipid Kinases; PKA/PKC Pathway

References

1. Imai, S. et al: Identification and characterization of a novel human type II diacylglycerol kinase, DGK kappa. J Biol Chem. 2005; 280(48):39870-81. 2. Buckland, A.G. et al: Anionic phospholipids, interfacial binding and the regulation of cell functions. Biochim Biophys Acta. 2000; 1483(2):199-216. 3. Xie, H. et al: Association between diacylglycerol kinase kappa variants and hypospadias susceptibility in a Han Chinese population. Asian journal of andrology. 2018; 20 (1): 85-89.

NCBI and Uniprot Product Information

• NCBI GI #: 62000702
• NCBI GeneID: 139189
• NCBI Accession #: NP_001013764.1
• NCBI GenBank Nucleotide #: NM_001013742.2
• UniProt Accession #: Q5KSL6; B2RP91
• Molecular Weight: 141,829 Da
• NCBI Official Full Name: diacylglycerol kinase kappa
• NCBI Official Synonym Full Names: diacylglycerol kinase, kappa
• NCBI Official Symbol: DGKK
• NCBI Protein Information: diacylglycerol kinase kappa; DGK-kappa; DAG kinase kappa; diglyceride kinase kappa; 142 kDa diacylglycerol kinase
• UniProt Protein Name: Diacylglycerol kinase kappa
• Protein Family: Diacylglycerol kinase
• UniProt Gene Name: DGKK
• UniProt Synonym Gene Names: DAG kinase kappa; DGK-kappa
• UniProt Entry Name: DGKK_HUMAN

NCBI Description

The protein encoded by this gene is an enzyme that phosphorylates diacylglycerol, converting it to phosphatidic acid. The encoded protein is a membrane protein and is inhibited by hydrogen peroxide. Variations in this gene have been associated with hypospadias. [provided by RefSeq, Mar 2011]

Uniprot Description

DGKK: Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Belongs to the eukaryotic diacylglycerol kinase family.

Protein type: Kinase, lipid; EC 2.7.1.107

Chromosomal Location of Human Ortholog: Xp11.22

Cellular Component: cytoplasm; plasma membrane

Molecular Function: metal ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: platelet activation; protein kinase C activation; diacylglycerol metabolic process; response to oxidative stress; blood coagulation; phosphorylation