DGKI recombinant protein

DGKI, Active

Cat. Num. AAA516805

• Gene Names: DGKI; DGK-IOTA
• Synonyms: DGKI; Active; Diacylglycerol kinase iota; DGK-iota; DGKI recombinant protein

• Host: Sf9 insect cells
• Form/Format: Recombinant protein stored in 50mM sodium phosphate, pH7.0, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.

• Tag: HIS
• Species: Human
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Activity

(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet)

SDS-Page

(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)

Related Product Information for DGKI recombinant protein

Recombinant full-length human DGKI was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. The gene accession number is NP_004708.1.
Diacylglycerol kinase iota (DGKI) is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases modulate the concentration of intracellular diacylglycerol by phosphorylating, producing phosphatidic acid (PA)(1). PA regulates signaling proteins, such as protein kinases and phosphatases, and can induce cell mitosis through regulation of growth factors (2). DGKI has been shown to overexpress in a variety of cancers and is associated with poor prognosis in colon and gastric cancer (3).

Product Categories/Family for DGKI recombinant protein

Lipid Kinases; Angiogenesis; Apoptosis/Autophagy; Cancer; Cardiovascular Disease; Cell Cycle; Inflammation; PKA/PKC Pathway

References

1. Ding, L. et al: The cloning and characterization of a novel human diacylglycerol kinase, DGKiota. J Biol Chem. 1998; 273(49):32746-52. 2. Buckland, AG. et al: Anionic phospholipids, interfacial binding and the regulation of cell functions. Biochim Biophys Acta. 2000; 1483(2):199-216. 3. Penrose, HM. et al: In colonic cells reduced mitochondrial function mediates transcriptomic alterations associated with cancer. Oncoscience. 2017; 4(11-12):189-198.

NCBI and Uniprot Product Information

• NCBI GI #: 4758156
• NCBI GeneID: 9162
• NCBI Accession #: NP_004708.1
• NCBI GenBank Nucleotide #: NM_004717.2
• UniProt Accession #: O75912; Q9NZ49; A4D1Q9
• Molecular Weight: 116,997 Da
• NCBI Official Full Name: diacylglycerol kinase iota
• NCBI Official Synonym Full Names: diacylglycerol kinase, iota
• NCBI Official Symbol: DGKI
• NCBI Official Synonym Symbols: DGK-IOTA
• NCBI Protein Information: diacylglycerol kinase iota; DAG kinase iota; diglyceride kinase iota
• UniProt Protein Name: Diacylglycerol kinase iota
• Protein Family: Diacylglycerol kinase
• UniProt Gene Name: DGKI
• UniProt Synonym Gene Names: DAG kinase iota; DGK-iota
• UniProt Entry Name: DGKI_HUMAN

NCBI Description

This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. [provided by RefSeq, Jul 2008]

Uniprot Description

DGKI: a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. [provided by RefSeq, Jul 2008]

Protein type: Kinase, lipid; Motility/polarity/chemotaxis; EC 2.7.1.107; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - glycerolipid

Chromosomal Location of Human Ortholog: 7q32.3-q33

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic spine; synapse; nucleus

Molecular Function: protein binding; Ras GTPase binding; metal ion binding; diacylglycerol kinase activity; ATP binding; GTPase inhibitor activity; NAD+ kinase activity

Biological Process: platelet activation; protein kinase C activation; negative regulation of catalytic activity; positive regulation of Ras protein signal transduction; blood coagulation; phosphorylation