Mouse Cartilage intermediate layer protein 1 (CILP) ELISA Kit | CILP elisa kit

Mouse Cartilage intermediate layer protein 1 (CILP) ELISA Kit

Cat. Num. AAA7200072

• Gene Names: CILP; CILP-1; HsT18872
• Reactivity: Mouse
• Synonyms: Cartilage intermediate layer protein 1 (CILP); Mouse Cartilage intermediate layer protein 1 (CILP) ELISA Kit; CILP elisa kit

• Reactivity: Mouse

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CILP elisa kit

Intended Uses: This CII ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken CII. This ELISA kit for research use only!

Principle of the Assay: CII ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CII antibody and an CII-HRP conjugate. The assay sample and buffer are incubated together with CII-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CII concentration since CII from samples and CII-HRP conjugate compete for the anti-CII antibody binding site. Since the number of sites is limited, as more sites are occupied by CII from the sample, fewer sites are left to bind CII-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CII concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CILP elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 317373560
• NCBI GeneID: 8483
• Molecular Weight: 132,565 Da
• NCBI Official Full Name: Cartilage intermediate layer protein 1
• NCBI Official Synonym Full Names: cartilage intermediate layer protein, nucleotide pyrophosphohydrolase
• NCBI Official Symbol: CILP
• NCBI Official Synonym Symbols: CILP-1; HsT18872
• NCBI Protein Information: cartilage intermediate layer protein 1; cartilage intermediate-layer protein; cartilage intermediate layer protein 1 C1; cartilage intermediate layer protein 1 C2
• UniProt Protein Name: Cartilage intermediate layer protein 1
• Protein Family: Cartilage intermediate layer protein
• UniProt Gene Name: CILP
• UniProt Synonym Gene Names: UNQ602/PRO1188; CILP-1
• UniProt Entry Name: CILP1_HUMAN

NCBI Description

Major alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. This gene encodes the cartilage intermediate layer protein (CILP), which increases in early osteoarthrosis cartilage. The encoded protein was thought to encode a protein precursor for two different proteins; an N-terminal CILP and a C-terminal homolog of NTPPHase, however, later studies identified no nucleotide pyrophosphatase phosphodiesterase (NPP) activity. The full-length and the N-terminal domain of this protein was shown to function as an IGF-1 antagonist. An allelic variant of this gene has been associated with lumbar disc disease. [provided by RefSeq, Sep 2010]

Uniprot Description

CILP: Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage. Defects in CILP are a cause of susceptibility to intervertebral disk disease (IDD). A common musculo- skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain. Susceptibility to intervertebral disk disease, is conferred by variant p.Ile395Thr (PubMed:15864306).

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix

Molecular Function: nucleotide diphosphatase activity; alkaline phosphatase activity

Biological Process: negative regulation of insulin-like growth factor receptor signaling pathway

Disease: Intervertebral Disc Disease