Rabbit anti-Human Periplakin Polyclonal Antibody | anti-PPL antibody

Periplakin, CT (PPL, Paraneoplastic Pemphigus Antigen, 195kD Cornified Envelope Precursor, AW553870, KIAA0568, MGC134872) (Azide free) (HRP)

Cat. Num. AAA6336361

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Periplakin; Polyclonal Antibody; CT (PPL; Paraneoplastic Pemphigus Antigen; 195kD Cornified Envelope Precursor; AW553870; KIAA0568; MGC134872) (Azide free) (HRP); PPL; 190kD paraneoplastic pemphigus antigen; 195kD cornified envelope precursor protein; anti-PPL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PPL antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1300-1331 amino acids from the C-terminal region of human PPL.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PPL Antibody (C-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the PPL antibody detected the PPL protein (arrow).)

Immunohistochemistry (IHC)

(PPL Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PPL Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PPL antibody

The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling. [provided by RefSeq].

Product Categories/Family for anti-PPL antibody

Antibodies; Signal Transduction

References

Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010): Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Pyle, A.L., et al. Cardiovasc. Pathol. 19 (2), E13-E20 (2010): Huang, Y., et al. Arch. Dermatol. Res. 301(10):703-709(2009). Boczonadi, V., et al. Exp. Cell Res. 313(16):3579-3591(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 45439327
• NCBI GeneID: 5493
• NCBI Accession #: NP_002696.3
• NCBI GenBank Nucleotide #: NM_002705.4
• UniProt Accession #: O60437; O60314; O60454; Q14C98
• Molecular Weight: 204,747 Da
• NCBI Official Full Name: periplakin
• NCBI Official Synonym Full Names: periplakin
• NCBI Official Symbol: PPL
• NCBI Protein Information: periplakin
• UniProt Protein Name: Periplakin
• Protein Family: Periplakin
• UniProt Gene Name: PPL
• UniProt Synonym Gene Names: KIAA0568
• UniProt Entry Name: PEPL_HUMAN

NCBI Description

The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling. [provided by RefSeq, Jul 2008]

Uniprot Description

periplakin: Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling. Belongs to the plakin or cytolinker family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: desmosome; cytoskeleton; mitochondrion; plasma membrane; nucleus

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: keratinization