Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
DGKZ recombinant protein
DGKZ, Active
Applications
Kinase Assay
Synonyms
DGKZ; Active; DAGK5; DAGK6; DGK-ZETA; hDGKzeta; DGKZ recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, and 25% glycerol.
Applicable Applications for DGKZ recombinant protein
Kinase Assay
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for DGKZ recombinant protein
Recombinant full-length human DGKZ was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The DGKZ gene accession number is NM_003646.
Diacylglycerol kinase zeta (DAG kinase zeta) is a member of class IV diacylglycerol kinase family, which contains two zinc fingers, an ATP binding site, and four ankyrin repeats with the MARCKS phosphorylation site, and exhibits the preference for 1,2-diacylglycerols but not for long chain diacylglycerols (1). DGK zeta regulates Ras activation via terminating the diacylglycerol signaling induced RasGRP (2). DGK zeta deficient mice were hyper-responsive to T cell receptor stimulation (3). Also, increased expression levels of DGK zeta promotes Rho GTPase activity and cancer cell invasion (4).
Diacylglycerol kinase zeta (DAG kinase zeta) is a member of class IV diacylglycerol kinase family, which contains two zinc fingers, an ATP binding site, and four ankyrin repeats with the MARCKS phosphorylation site, and exhibits the preference for 1,2-diacylglycerols but not for long chain diacylglycerols (1). DGK zeta regulates Ras activation via terminating the diacylglycerol signaling induced RasGRP (2). DGK zeta deficient mice were hyper-responsive to T cell receptor stimulation (3). Also, increased expression levels of DGK zeta promotes Rho GTPase activity and cancer cell invasion (4).
Product Categories/Family for DGKZ recombinant protein
References
1. Bunting, M. et al. Molecular Cloning and Characterization of a Novel Human Diacylglycerol Kinase zeta. The Journal of Biological Chemistry. (271) 10230-10236, 1996.
2. Topham, M. et al. Diacylglycerol Kinase zeta Regulates Ras Activation by a Novel Mechanism. The Journal of Cell Biology. (152) 1135-1143, 2001.
3. Zhong X. et al. Enhanced T cell responses due to diacylglycerol kinase zeta deficiency. Nature Immunology. (4) 882-890, 2003.
4. Cai K. et al. Increased diacylglycerol kinase zeta expression in human metastatic colon cancer cells augments Rho GTPase activity and contributes to enhanced invasion. BMC Cancer. (14) 208-217, 2014.
2. Topham, M. et al. Diacylglycerol Kinase zeta Regulates Ras Activation by a Novel Mechanism. The Journal of Cell Biology. (152) 1135-1143, 2001.
3. Zhong X. et al. Enhanced T cell responses due to diacylglycerol kinase zeta deficiency. Nature Immunology. (4) 882-890, 2003.
4. Cai K. et al. Increased diacylglycerol kinase zeta expression in human metastatic colon cancer cells augments Rho GTPase activity and contributes to enhanced invasion. BMC Cancer. (14) 208-217, 2014.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
132kDa
Protein Family
Similar Products
Product Notes
The DGKZ (Catalog #AAA516809) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DGKZ can be used in a range of immunoassay formats including, but not limited to, Kinase Assay. Researchers should empirically determine the suitability of the DGKZ for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DGKZ, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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