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124 results for " Lipid Kinases" - showing 1-50


Western Blot (WB) (Western blot detection of LCK in Jurkat and Ramos cell lysates and using LCK mouse mAb (1:1000 diluted).Predicted band size: 58KDa.Observed band size: 58KDa.)

LCK, Monoclonal Antibody (Cat# AAA475086)

Full Name
Anti-LCK Mouse mAb
Gene Names
LCK; LSK; YT16; IMD22; p56lck; pp58lck
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (Western blot detection of PI3 Kinase p85 beta in C6, K562, MCF7, U87MG, HCT116, 3T3 and Hela cell lysates using PI3 Kinase p85 beta mouse mAb (1:1000 diluted).Predicted band size:85KDa.Observed band size:85KDa.)

PI3 Kinase p85 beta, Monoclonal Antibody (Cat# AAA475225)

Full Name
Anti-PI3 Kinase p85 beta Mouse mAb
Gene Names
PIK3R2; p85; MPPH; P85B; MPPH1; p85-BETA
Reactivity
Human, Mouse, Rat
Applications
WB
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Immunohistochemistry (IHC) (Figure 5. IHC analysis of Lyn using anti-Lyn antibody (MBS1750642). Lyn was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Lyn Antibody (MBS1750642) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

Lyn, Polyclonal Antibody (Cat# AAA1750642)

Full Name
Anti-Lyn Picoband Antibody
Gene Names
LYN; JTK8; p53Lyn; p56Lyn
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data #1 (Paraffin embedded human brain (cerebellar cortex) stained with Goat anti human p110alpha (MBS223510))

PI-3 KINASE p110 SUBUNIT ALPHA, Polyclonal Antibody (Cat# AAA223510)

Full Name
GOAT ANTI HUMAN PI-3 KINASE p110 SUBUNIT ALPHA
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Bovine (Expected from Sequence), Dog (Expected from Sequence), Mouse (Expected from Sequence), Rat (Expected from Sequence)
Applications
EIA
Pricing
$625/0.1 mg | $2,670/5x0.1 mg
Dot Blot (DB) (Dot blot analysis of anti-Phospho-INSR-pY1185 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Phospho-INSR (Y1185), Polyclonal Antibody (Cat# AAA9204291)

Full Name
Phospho-INSR (Y1185) Antibody
Gene Names
INSR; HHF5; CD220
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat, Xenopus, Drosophila)
Applications
DB, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Western blot detection of Fyn in Hela, 3T3, C6, COS7, CEM, Ramos and Jurkat cell lysates using Fyn mouse mAb (1:500 diluted).Predicted band size:59KDa.Observed band size:59KDa.)

Fyn, Monoclonal Antibody (Cat# AAA475169)

Full Name
Anti-Fyn Mouse mAb
Gene Names
FYN; SLK; SYN; p59-FYN
Reactivity
Human, Monkey, Rat, Mouse
Applications
WB, ICC
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Immunohistochemistry (IHC) (Immunohistochemical staining of normal human liver tissue using CERT antibody at 15 ug/ml)

Ceramide Transfer Protein (CERTL), Polyclonal Antibody (Cat# AAA395799)

Full Name
Ceramide Transfer Protein (CERTL)
Gene Names
COL4A3BP; CERT; GPBP; CERTL; STARD11
Reactivity
Human
Applications
WB
Purity
Antigen Immunoaffiinity Purification
Pricing
$390/0.1 mg | $1,610/5x0.1 mg

Ceramide Transfer Protein (CERTL), Peptide (Cat# AAA396460)

Full Name
Ceramide Transfer Protein (CERTL)
Gene Names
COL4A3BP; CERT; GPBP; CERTL; STARD11
Reactivity
Human
Applications
WB
Pricing
$240/0.05 mg | $925/5x0.05 mg
Western Blot (WB)

FGR, Polyclonal Antibody (Cat# AAA540827)

Full Name
FGR Antibody Affinity Purified
Gene Names
FGR; SRC2; c-fgr; c-src2; p55-Fgr; p58-Fgr; p55c-fgr; p58c-fgr
Reactivity
Human, Mouse, Rat
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Western Blot (WB) (Western blot analysis of PI3KC2G antibody (N-term) in CEM cell line lysates (35ug/lane). PI3KC2G (arrow) was detected using the purified Pab.)

PI3KC2G, Polyclonal Antibody (Cat# AAA9205951)

Full Name
PI3KC2G Antibody (N-term)
Gene Names
PIK3C2G; PI3K-C2GAMMA; PI3K-C2-gamma
Reactivity
Human
Applications
WB, EIA, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Specific Activity (The specific activity of PIP5K1C was determined to be 6,927 nmol/min/mg as per activity assay protocol.)

PIP5K1C, Active Protein (Cat# AAA515572)

Full Name
PIP5K1C, Active
Gene Names
PIP5K1C; LCCS3; PIP5Kgamma; PIP5K-GAMMA; PIP5K1-gamma
Purity
>80%
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Western Blot/Blotting (Western blot using CERTL antibody on HT-29 cell lysate (28 ug/lane). Primary antibody used at 10ug/ml. Secondary antibody, mouse anti-rabbit HRP , used at 1:50k.)

Ceramide Transfer Protein (CERTL), Polyclonal Antibody (Cat# AAA395899)

Full Name
Ceramide Transfer Protein (CERTL)
Gene Names
COL4A3BP; CERT; GPBP; CERTL; STARD11
Reactivity
Human
Applications
WB
Purity
Ammonium Sulfate Precipitation
Pricing
$390/0.1 mg | $1,610/5x0.1 mg

Ceramide Kinase, Polyclonal Antibody (Cat# AAA395700)

Full Name
Ceramide Kinase
Gene Names
CERK; LK4; hCERK; dA59H18.2; dA59H18.3
Reactivity
Human
Applications
WB, EIA
Purity
Ammonium Sulfate Precipitation
Pricing
$390/0.1 mg | $1,610/5x0.1 mg

Protein Kinase C, gamma, Recombinant Protein (Cat# AAA636563)

Full Name
Protein Kinase C, gamma, Recombinant, Human (PKCg)
Gene Names
PRKCG; PKCC; PKCG; SCA14; PKC-gamma
Purity
Highly Purified
90% (SDS-PAGE)
Pricing
$515/0.01 mg | $365/0.002 mg | $2,305/5x0.01 mg
Western Blot (WB) (The anti-CERK Pab is used in Western blot to detect CERK in mouse heart tissue lysate (Lane 1) and A2058 cell lysate (Lane 2).)

CERK, Polyclonal Antibody (Cat# AAA641552)

Full Name
CERK, CT (CERK, KIAA1646, Ceramide kinase, Acylsphingosine kinase, Lipid kinase 4)
Gene Names
CERK; LK4; hCERK; dA59H18.2; dA59H18.3
Reactivity
Human, Mouse
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL
Testing Data

PI3K, Recombinant Protein (Cat# AAA515587)

Full Name
PI3K (p110 alpha (E542K)/p85 alpha, Active
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; PI3K-alpha; p110-alpha
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Testing Data

PIP5K1B, Recombinant Protein (Cat# AAA515571)

Full Name
PIP5K1B, Active
Gene Names
PIP5K1B; MSS4; STM7
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Testing Data

PI3K, Recombinant Protein (Cat# AAA515584)

Full Name
PI3K (p110 alpha/p85 alpha), Active
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; PI3K-alpha; p110-alpha
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Western Blot (WB) (Western blot detection of Lck in Jurkat and Ramos cell lysates and using Lck mouse mAb (1:1000 diluted).Predicted band size: 56KDa.Observed band size: 56KDa.)

Lck, Monoclonal Antibody (Cat# AAA475701)

Full Name
Anti-Lck Mouse mAb
Gene Names
LCK; LSK; YT16; IMD22; p56lck; pp58lck
Reactivity
Human
Applications
WB
Pricing
$440/0.1 mL | $1,520/5x0.1 mL

Protein Kinase C, iota/lamda Serine/Threonine Kinase, full length, Active Protein (Cat# AAA637650)

Full Name
Protein Kinase C, iota/lamda Serine/Threonine Kinase, Recombinant, Human, full length (PKCI)
Reactivity
Human
Purity
>95% by SDS-PAGE.
Pricing
$675/0.01 mg | $3,040/5x0.01 mg
Testing Data

SPHK1, Recombinant Protein (Cat# AAA515664)

Full Name
SPHK1, Active
Gene Names
SPHK1; SPHK
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Testing Data (The specific activity of PIP5K1A was determined to be 4,000 nmol/min/mg as per activity assay protocol.)

PIP5K1A, Recombinant Protein (Cat# AAA515570)

Full Name
PIP5K1A, Active
Purity
>85%
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Western Blot (WB) (Western blot analysis of Lyn phosphorylation expression in Jurkat cell lysate treated with Pervanadate (MBS1750264).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYN monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LYN )

Lyn, Monoclonal Antibody (Cat# AAA1750264)

Full Name
Anti-Phospho-Lyn (Y396) Rabbit Monoclonal Antibody
Gene Names
LYN; JTK8; p53Lyn; p56Lyn
Reactivity
Human, Mouse
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Testing Data

PIP4K2A, Recombinant Protein (Cat# AAA515619)

Full Name
PIP4K2A, Active
Gene Names
PIP4K2A; PIPK; PI5P4KA; PIP5K2A; PIP5KIIA; PIP5KII-alpha
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Testing Data

PI3K, Recombinant Protein (Cat# AAA515590)

Full Name
PI3K (p110 beta/p85 beta), Active
Gene Names
PIK3CB; PI3K; PIK3C1; P110BETA; PI3KBETA
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Western Blot (WB) (Western blot analysis of Phospho-PTEN (S380) expression in (1) MCF-7 cell treated with alkaline phosphatase lysate; (2) MCF-7 cell lysate (MBS1750177).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTEN monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PTEN )

PTEN, Monoclonal Antibody (Cat# AAA1750177)

Full Name
Anti-Phospho-PTEN (S380) Rabbit Monoclonal Antibody
Gene Names
PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta
Reactivity
Human, Mouse, Rat
Applications
IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Testing Data

PI3K, Recombinant Protein (Cat# AAA515588)

Full Name
PI3K (p110 alpha (E545K)/p85 alpha, Active
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; PI3K-alpha; p110-alpha
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Testing Data

PI3K, Recombinant Protein (Cat# AAA515586)

Full Name
PI3K (p110 alpha (N345K (/p85 alpha), Active
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; PI3K-alpha; p110-alpha
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Testing Data

PI4K2A, Recombinant Protein (Cat# AAA515576)

Full Name
PI4K2A, Active
Gene Names
PI4K2A; PI4KII; PIK42A
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Testing Data

PIP5K3, Recombinant Protein (Cat# AAA515574)

Full Name
PIP5K3 (PIKFYVE), Active
Gene Names
PIKFYVE; CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Testing Data

PI4KA, Recombinant Protein (Cat# AAA515594)

Full Name
PI4KA, Active
Gene Names
PI4KA; PIK4CA; PMGYCHA; pi4K230; PI4K-ALPHA
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Testing Data

PIK3C2B, Recombinant Protein (Cat# AAA515579)

Full Name
PIK3C2B, Active
Gene Names
PIK3C2B; C2-PI3K
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Western Blot (WB) (Western blot analysis of PTEN expression in Hela cell lysate (MBS179044).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTEN monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PTEN )

PTEN, Monoclonal Antibody (Cat# AAA179044)

Full Name
Anti-PTEN Rabbit Monoclonal Antibody
Gene Names
PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Testing Data

PIK3C3, Recombinant Protein (Cat# AAA515581)

Full Name
PIK3C3 (Vps34), Active
Gene Names
PIK3C3; VPS34; Vps34; hVps34
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Testing Data

PI3K, Recombinant Protein (Cat# AAA515582)

Full Name
PI3K (p110 alpha/p55 gamma), Active
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; PI3K-alpha; p110-alpha
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Western Blot (WB) (Western blot analysis of extracts of MCF-7 cell lysate, using PTEN antibody (MBS179043).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTEN monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PTEN )

PTEN, Monoclonal Antibody (Cat# AAA179043)

Full Name
Anti-PTEN Rabbit Monoclonal Antibody
Gene Names
PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Testing Data

PI3K, Recombinant Protein (Cat# AAA515589)

Full Name
PI3K (p110 alpha/p85 alpha), Active
Gene Names
Pik3ca; p110; caPI3K; p110alpha; 6330412C24Rik
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Testing Data

PIP4K2B, Recombinant Protein (Cat# AAA515620)

Full Name
PIP4K2B, Active
Gene Names
PIP4K2B; PI5P4KB; PIP5K2B; PIP5KIIB; PIP5P4KB; PIP5KIIbeta
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Testing Data

PI3K, Recombinant Protein (Cat# AAA515583)

Full Name
PI3K (p110 alpha/p85 alpha), Active
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; PI3K-alpha; p110-alpha
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Testing Data

PIK3C2A, Recombinant Protein (Cat# AAA515578)

Full Name
PIK3C2A, Active
Gene Names
PIK3C2A; CPK; PI3-K-C2A; PI3K-C2alpha; PI3K-C2-alpha; PI3-K-C2(ALPHA)
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Testing Data

PI3K, Recombinant Protein (Cat# AAA515593)

Full Name
PI3K (p110 delta/p85 alpha), Active
Gene Names
PIK3CD; APDS; PI3K; IMD14; p110D; P110DELTA
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Western Blot (WB) (Western blot analysis of AMPK beta 1 expression in HeLa cell lysate (MBS179983).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAB1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRKAB1 )

AMPK beta 1, Monoclonal Antibody (Cat# AAA179983)

Full Name
Anti-AMPK beta 1 Rabbit Monoclonal Antibody
Gene Names
PRKAB1; AMPK; HAMPKb
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Testing Data

PI3K, Recombinant Protein (Cat# AAA515592)

Full Name
PI3K (p120 gamma), Active
Gene Names
PIK3CG; PI3K; PIK3; PI3CG; PI3Kgamma; p110gamma; p120-PI3K
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Testing Data

PIP4K2C, Recombinant Protein (Cat# AAA515621)

Full Name
PIP4K2C, Active
Gene Names
PIP4K2C; PIP5K2C
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human astrocytoma, using PI 3 Kinase p55 gamma Antibody(MBS1750083)PIK3R3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PIK3R3 Antibody (MBS1750083)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

PI 3 Kinase p55 gamma, Monoclonal Antibody (Cat# AAA1750083)

Full Name
Anti-PI 3 Kinase p55 gamma Rabbit Monoclonal Antibody
Gene Names
PIK3R3; p55; p55PIK; p55-GAMMA
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of FGFR2 expression in (1) MCF-7 cell lysate; (2) Mouse brain lysate (MBS179209).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FGFR2 )

FGFR2, Monoclonal Antibody (Cat# AAA179209)

Full Name
Anti-FGFR2 Rabbit Monoclonal Antibody
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Reactivity
Human, Mouse, Rat
Applications
IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Testing Data

SPHK2, Recombinant Protein (Cat# AAA515665)

Full Name
SPHK2, Active
Gene Names
SPHK2; SK 2; SK-2; SPK 2; SPK-2
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Testing Data

PI4K2B, Recombinant Protein (Cat# AAA515577)

Full Name
PI4K2B, Active
Gene Names
PI4K2B; PIK42B; PI4KIIB
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Testing Data

PI3K, Recombinant Protein (Cat# AAA515591)

Full Name
PI3K (p110 beta/p85 alpha), Active
Gene Names
PIK3CB; PI3K; PIK3C1; P110BETA; PI3KBETA
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
Testing Data

PIK3C2G, Recombinant Protein (Cat# AAA515580)

Full Name
PIK3C2G, Active
Gene Names
PIK3C2G; PI3K-C2GAMMA; PI3K-C2-gamma
Applications
Kinase Assay
Pricing
$435/0.01 mg | $335/0.005 mg | $1,735/5x0.01 mg
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