Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
DGKE recombinant protein
DGKE, Active
Applications
Kinase Assay
Synonyms
DGKE; Active; DGK; AHUS7; DAGK5; DAGK6; NPHS7; DGKE recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, and 25% glycerol.
Applicable Applications for DGKE recombinant protein
Kinase Assay
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for DGKE recombinant protein
Recombinant full-length human DGKE was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The DGKE gene accession number is NM_003647.
Diacylglycerol kinase epsilon (DAG kinase epsilon) is the only member of class III diacylglycerol kinase family, which lacks the E-F hand motifs for Ca+ binding but possess a clear selectivity for arachidonoyl-containing species of diacylglycerol (1). DGK epsilon might decrease signals conducted via arachidonoyl-diacylglycerol or promotes the synthesis of phospholipids with certain fatty acids (1). DGK epsilon contributes to Huntington disease pathogenesis and its inhibition might be effect in the therapeutics (2). Also, recessive mutations in DGKE results in unrestricted complement activation in atypical hemolytic-uremic syndrome (3).
Diacylglycerol kinase epsilon (DAG kinase epsilon) is the only member of class III diacylglycerol kinase family, which lacks the E-F hand motifs for Ca+ binding but possess a clear selectivity for arachidonoyl-containing species of diacylglycerol (1). DGK epsilon might decrease signals conducted via arachidonoyl-diacylglycerol or promotes the synthesis of phospholipids with certain fatty acids (1). DGK epsilon contributes to Huntington disease pathogenesis and its inhibition might be effect in the therapeutics (2). Also, recessive mutations in DGKE results in unrestricted complement activation in atypical hemolytic-uremic syndrome (3).
Product Categories/Family for DGKE recombinant protein
References
1. Tang, W. et al. Molecular Cloning of a Novel Human Diacylglycerol Kinase Highly Selective for Arachidonate-containing Substrates. The Journal of Biological Chemistry. (271) 10237-10241, 1996.
2. Zhang, N. et al. Inhibition of Lipid Signaling Enzyme Diacylglycerol Kinase Epsilon Attenuates Mutant Huntingtin Toxicity. The Journal of Biological Chemistry. (287) 21204-21213, 2012.
3. Lemaire, M. et al. Recessive mutations in DGKE cause atypical hemolytic-uremic syndrome. Nature Genetics. (45) 531-536, 2013.
2. Zhang, N. et al. Inhibition of Lipid Signaling Enzyme Diacylglycerol Kinase Epsilon Attenuates Mutant Huntingtin Toxicity. The Journal of Biological Chemistry. (287) 21204-21213, 2012.
3. Lemaire, M. et al. Recessive mutations in DGKE cause atypical hemolytic-uremic syndrome. Nature Genetics. (45) 531-536, 2013.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
91kDa
Protein Family
Similar Products
Product Notes
The DGKE (Catalog #AAA516796) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DGKE can be used in a range of immunoassay formats including, but not limited to, Kinase Assay. Researchers should empirically determine the suitability of the DGKE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DGKE, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
