Mouse Collagen Type X ELISA Kit | COL10 elisa kit

Mouse Collagen Type X ELISA Kit

Cat. Num. AAA729640

• Reactivity: Mouse
• Synonyms: Collagen Type X; Mouse Collagen Type X ELISA Kit; COL10 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This COL10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse COL10. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL10 elisa kit

Principle of the assay: COL10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-COL10 antibody and an COL10-HRP conjugate. The assay sample and buffer are incubated together with COL10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COL10 concentration since COL10 from samples and COL10-HRP conjugate compete for the anti-COL10 antibody binding site. Since the number of sites is limited, as more sites are occupied by COL10 from the sample, fewer sites are left to bind COL10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COL10 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for COL10 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 18105032
• NCBI GeneID: 1300
• NCBI Accession #: NP_000484.2
• NCBI GenBank Nucleotide #: NM_000493.3
• Molecular Weight: 66,158 Da
• NCBI Official Full Name: collagen alpha-1(X) chain
• NCBI Official Synonym Full Names: collagen, type X, alpha 1
• NCBI Official Symbol: COL10A1
• NCBI Protein Information: collagen alpha-1(X) chain; collagen alpha-1(X) chain; collagen X, alpha-1 polypeptide; Schmid metaphyseal chondrodysplasia
• UniProt Protein Name: Collagen alpha-1(X) chain
• Protein Family: Zinc finger protein CONSTANS
• UniProt Gene Name: COL10A1
• UniProt Entry Name: COAA1_HUMAN

NCBI Description

This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD). [provided by RefSeq, Jul 2008]

Uniprot Description

COL10A1: Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage. Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD). SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6q21-q22

Cellular Component: proteinaceous extracellular matrix; collagen; endoplasmic reticulum lumen; extracellular region; cell cortex

Molecular Function: metal ion binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; cartilage development; skeletal development; endochondral ossification

Disease: Metaphyseal Chondrodysplasia, Schmid Type