Rabbit ULP1 Polyclonal Antibody | anti-ULP1 antibody

Anti-Yeast ULP1 Antibody

Cat. Num. AAA4156663

• Gene Names: Ulp1; BcDNA:GH02751; CG12359; DmelCG12359; DmUlp1; ulp1
• Applications: Western Blot, ELISA
• Purity: Affinity Purified
• Synonyms: ULP1; Polyclonal Antibody; Anti-Yeast ULP1 Antibody; Yeast ULP1; anti-ULP1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Reactivity against ULP-1 from other sources or ULP-2 has not been determined.
• Purity/Purification: Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 1513

• Applicable Applications for anti-ULP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: This purified polyclonal antibody reacts with yeast ULP-1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 72.4 kDa in size corresponding to yeast ULP-1 by western blotting in the appropriate lysate or extract.
• Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast ULP-1 protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Testing Data

Related Product Information for anti-ULP1 antibody

ULP-1, ubiquitin-like protein-specific protease 1, initially processes Smt3 and also acts as a deconjugating enzyme for Smt3 [Saccharomyces cerevisiae (Baker's yeast)]. Covalent modification of cellular proteins by the ubiquitin-like modifier SUMO (small ubiquitin-like modifier) regulates various cellular processes, such as nuclear transport, signal transduction, stress responses and cell cycle progression. But, in contrast to ubiquination, sumoylation does not tag proteins for degradation by the 26S proteasome, but rather seems to enhance stability or modulate their subcellular compartmentalization. Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA interactions, as well as localization and stability of the target protein. Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to humans. Where invertebrates have only a single SUMO gene termed SMT3, three members of the SUMO family have been identified in vertebrates: SUMO-1 and the close homologues SUMO-2 and SUMO-3. Three distinct steps can be distinguished in the SUMO modification pathway: 1) activation of SUMO, 2) transfer of SUMO to the conjugating enzyme, and 3) substrate modification. Since SUMO is synthesized as a precursor protein, a maturation step precedes the activation reaction. In yeast, C-terminal processing of the SUMO precursor is mediated by the processing protease Ulp1, which has an additional role in the deconjugation of SUMO-modified substrates. Mature SUMO is activated by SUMO-activating enzyme, an E1-like heterodimeric protein complex composed of Uba2 and Aos1. Ulp1 function has provided evidence that SUMO modification in yeast, as has been suspected for vertebrates, plays an important role in nucleocytoplasmic trafficking.

NCBI and Uniprot Product Information

• NCBI GI #: 18860521
• NCBI GeneID: 32910
• NCBI GenBank Nucleotide #: NP_573362.1
• NCBI Official Full Name: Ulp1
• NCBI Official Symbol: Ulp1
• NCBI Official Synonym Symbols: BcDNA:GH02751; CG12359; DmelCG12359; DmUlp1; ulp1
• NCBI Protein Information: Ulp1
• Protein Family: ULP1-interacting protein