Rat CD8 Monoclonal Antibody | anti-CD8 antibody

RAT ANTI DOG CD8:FITC

Cat. Num. AAA214752

• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD8; Monoclonal Antibody; RAT ANTI DOG CD8:FITC; anti-CD8 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: YCATE55.9
• Form/Format: FITC; Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)
• Sequence Length: 239

• Applicable Applications for anti-CD8 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/5
• Perservative Stabilisers: 0.09% Sodium Azide; 1% Bovine Serum Albumin; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Dog
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of canine peripheral blood lymphocytes with Rat anti Canine CD8:FITC)

Testing Data

(Staining of canine peripheral blood lymphocytes with Rat anti Dog CD8 (MBS224414))

Testing Data

(Published customer image: CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)

Testing Data

(Published customer image:Survival rates of animals with canine mammary carcinoma. Kaplan-Meier survival curves for animals for All (MC-BMT and MC) animals categorized according to the relative percentage of CD4+ T-cells (33.3%) (A). Animals were submitted to quarterly follow-ups during twelve months and survival rates expressed in days between the surgical excisions of the end of follow-up as described in Methods. The survival curves were estimated with the Kaplan-Meier method followed by Log-rank test. Correlation analysis highlighted the significant association between the percentages of CD4+ T-cells and CD8+ T-cells with the animal survival in days (B).From: Estrela-Lima et al. BMC Cancer 2010 10:256.)

Testing Data

(Published customer image: Immunophenotypic profile of tumor infiltrating lymphocyte in canine mammary carcinomas. Analysis of tumor infiltrating T-cells, B-lymphocytes and T-cell subsets from MC-BMT or MC (A), further subcategorized according to the absence (-) or presence (+) of lymph node metastasis (-) (B). Lymphocyte populations and subsets were identified by flow cytometric immunostaining as described in Material and Methods. Data were expressed as percentage of positive cells within gated lymphocytes and CD4+/CD8+ T-cell ratio. Significant differences at p < 0.05 are highlighted by asterisk.From: Estrela-Lima et al. BMC Cancer 2010 10:256.)

Testing Data

(Staining of canine peripheral blood lymphocytes with Rat anti Canine CD8:RPE (MBS214753))

Related Product Information for anti-CD8 antibody

Rat anti Dog CD8 antibody, clone YCATE55.9 was clustered as Canine CD8 in the First Canine Leukocyte Antigen Workshop (Cobbold et al. 1994). YCATE55.9 reacts with a rat cell line transfected with cDNA for canine CD8alpha (Gorman et al. 1994) and blocks MHC class I dependant T-cell responses in vitro and in vivo. Rat anti Dog CD8, clone YCATE55.9 has been shown to deplete circulating CD8+ T cells when administered to dogs in vivo. (Watson et al. 1993) Reduced levels of circulating CD8+ T cells has been associated with decreased survival times for dogs with osteosarcoma (Biller et al. 2010)

NCBI and Uniprot Product Information

• NCBI GI #: 55742790
• NCBI GeneID: 403157
• NCBI Accession #: NP_001002935.1
• NCBI GenBank Nucleotide #: NM_001002935.2
• UniProt Accession #: P33706
• Molecular Weight: 26,036 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD8 alpha chain
• NCBI Official Symbol: CD8A
• NCBI Protein Information: T-cell surface glycoprotein CD8 alpha chain; CD8 antigen, alpha polypeptide (p32)
• UniProt Protein Name: T-cell surface glycoprotein CD8 alpha chain
• Protein Family: CD81 antigen
• UniProt Gene Name: CD8A
• UniProt Entry Name: CD8A_CANFA

Uniprot Description

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.