Ubiquitin-Conjugating Enzyme E2 I Recombinant Protein | UBE2I recombinant protein

Recombinant Human Ubiquitin-Conjugating Enzyme E2 I Protein

Cat. Num. AAA2556613

• Gene Names: UBE2I; P18; UBC9; C358B7.1
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Ubiquitin-Conjugating Enzyme E2 I; Recombinant Human Ubiquitin-Conjugating Enzyme E2 I Protein; SUMO-Conjugating Enzyme UBC9; SUMO-Protein Ligase; Ubiquitin Carrier Protein 9; Ubiquitin Carrier Protein I; Ubiquitin-Protein Ligase I; p18; UBE2I; UBC9; UBCE9; UBE2I recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 mum filtered solution of 50mM HEPES, 150mM NaCl, pH 7.5.
• Sequence Length: 158

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fragment: Met1-Ser158
• Fusion Tag: N-GST
• Preparation and Storage: Store at < -20 degree C, stable for 6 months after receipt.; Please minimize freeze-thaw cycles.

SDS-Page

Related Product Information for UBE2I recombinant protein

Protein Construction: Recombinant Human Ubiquitin-Conjugating Enzyme E2 I is produced by our E Coli expression system and the target gene encoding Met1-Ser158 is expressed with a GST tag at the N-terminus.

Background: SUMO-Conjugating Enzyme UBC9 (UBC9) belongs to the ubiquitin-conjugating enzyme family. UBC9 is homologous to ubiquitin-conjugating enzymes (E2s). However, instead of conjugating ubiquitin, UBC9 conjugates a ubiquitin homologue, Small Ubiquitin-Like Modifier 1 (SUMO-1). The conjugation of ubiquitin requires the activities of ubiquitin-activating (E1) and conjugating (E2) enzymes. It is suggested that UBC9 might play a role in DNA repair and perhaps even in aging.

NCBI and Uniprot Product Information

• NCBI GI #: 4507785
• NCBI GeneID: 7329
• NCBI Accession #: NP_003336.1
• NCBI GenBank Nucleotide #: NM_003345.5
• UniProt Accession #: P63279; P50550; Q15698; Q59GX1; Q86VB3; D3DU69
• Molecular Weight: Molecular Mass: 44.4kDa; Actual Protein Molecular Mass: 40kDa
• NCBI Official Full Name: SUMO-conjugating enzyme UBC9
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 I
• NCBI Official Symbol: UBE2I
• NCBI Official Synonym Symbols: P18; UBC9; C358B7.1
• NCBI Protein Information: SUMO-conjugating enzyme UBC9
• UniProt Protein Name: SUMO-conjugating enzyme UBC9
• UniProt Gene Name: UBE2I
• UniProt Synonym Gene Names: UBC9; UBCE9

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'.