Human troponin I type 3 (cardiac) ELISA Kit | TNNI3 elisa kit

Human cardiac troponin I , cTn-I  ELISA Kit

Cat. Num. AAA700416

• Gene Names: TNNI3; CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
• Reactivity: Human
• Synonyms: troponin I type 3 (cardiac); Human cardiac troponin I ; cTn-I ELISA Kit; Human cardiac troponin I; cTn-IELISA Kit; CMD2A; CMH7; MGC116817; RCM1; TNNC1; cTnI; familial hypertrophic cardiomyopathy 7; troponin I; cardiac; TNNI3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human TNNI3. No significant cross-reactivity or interference between Human TNNI3 and analogues was observed.
• Sequence Length: 210

• Samples: Serum, plasma, urine
• Detection Range: 47 pg/ml-3000 pg/ml
• Sensitivity: 11.75 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNNI3 elisa kit

Introduction: Troponin I is a part of the troponin complex. It binds to actin in thinmyofilaments to hold the actin-tropomyosin complex in place. TnIinhibits ATPase activity of acto myosin and TnT and TnI arepresent in cardiac muscles in different forms than in skeletalmuscles. Only one tissue specific isoform of TnI is described forcardiac muscle tissue (cTnI) and this is expressed only inmyocardium. Equally reactive with free cardiac troponin I (cTnI)and cTnI forming complexes with other troponin components. (Inthe presence of 5 mM EDTA). Antibodies are not affected byheparin, phosphorylation, oxidation and troponin complexformation. No cross-reaction with skeletal muscle troponin I.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with anantibody specific to cTn-I. Standards or samples are then addedto the appropriate microtiter plate wells with a biotin-conjugatedantibody preparation specific for cTn-I and Avidin conjugated toHorseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine)substrate solution is added to each well. Only those wells thatcontain cTn-I, biotin-conjugated antibody and enzyme-conjugatedAvidin will exhibit a change in color. The enzyme-substrate reactionis terminated by the addition of a sulphuric acid solution and thecolor change is measured spectrophotometrically at a wavelengthof 450 nm +/- 2 nm. The concentration of cTn-I in the samples isthen determined by comparing the O.D. of the samples to thestandard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 151101270
• NCBI GeneID: 7137
• NCBI Accession #: NP_000354.4
• NCBI GenBank Nucleotide #: NM_000363.4
• UniProt Accession #: P19429
• Molecular Weight: 24,008 Da
• NCBI Official Full Name: troponin I, cardiac muscle
• NCBI Official Synonym Full Names: troponin I type 3 (cardiac)
• NCBI Official Symbol: TNNI3
• NCBI Official Synonym Symbols: CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
• NCBI Protein Information: troponin I, cardiac muscle; cardiomyopathy, dilated 2A (autosomal recessive)
• UniProt Protein Name: Troponin I, cardiac muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNI3
• UniProt Synonym Gene Names: TNNC1
• UniProt Entry Name: TNNI3_HUMAN

NCBI Description

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.