Rabbit Fra-1 Polyclonal Antibody | anti-Fra-1 antibody

Fra-1 (D134) Polyclonal Antibody

Cat. Num. AAA3001899

• Gene Names: FOSL1; FRA; FRA1; fra-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Fra-1; Polyclonal Antibody; Fra-1 (D134) Polyclonal Antibody; Fos-related antigen 1; FRA-1; FOSL1; FRA1; anti-Fra-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Fra-1 (D134) polyclonal antibody detects endogenous levels of Fra-1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 271

• Applicable Applications for anti-Fra-1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human Fra-1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Fra-1 (D134) pAb at 1:500 dilutionLane1:PC3 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:The Brain tissue lysate of Mouse (40ug) Lane4:PC12 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Fra-1 (D134) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Related Product Information for anti-Fra-1 antibody

The v-Fos oncogene was initially detected in two independent murine osteosarcoma virus isolates and an avian nephroblastoma virus. Members of the c-Fos gene family, including c-Fos, Fos B, Fra-1 and Fra-2, encode nuclear phosphoproteins that are rapidly and transiently induced by a variety of agents and function as transcriptional regulators for several genes. In contrast to c-Jun proteins, which form homo- and heterodimers that bind to specific DNA response elements, c-Fos proteins are only active as heterodimers with members of the Jun gene family. In addition, selected ATF/CREB family members can form leucine zipper dimers with Fos and Jun. Different dimers exhibit differential specificity and affinity for AP-1 and CRE sites.

NCBI and Uniprot Product Information

• NCBI GI #: 4885243
• NCBI GeneID: 8061
• NCBI Accession #: NP_005429.1
• NCBI GenBank Nucleotide #: NP_005429.1
• UniProt Accession #: P15407
• Molecular Weight: ~ 30, 42kDa
• NCBI Official Full Name: fos-related antigen 1 isoform 1
• NCBI Official Synonym Full Names: FOS like 1, AP-1 transcription factor subunit
• NCBI Official Symbol: FOSL1
• NCBI Official Synonym Symbols: FRA; FRA1; fra-1
• NCBI Protein Information: fos-related antigen 1
• UniProt Protein Name: Fos-related antigen 1
• UniProt Gene Name: FOSL1
• UniProt Synonym Gene Names: FRA1; FRA-1
• UniProt Entry Name: FOSL1_HUMAN

NCBI Description

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Uniprot Description

FRA1: an oncogenic transcription factor of the bZIP family, Fos subfamily. The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to DNA. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kinases in response to extracellular stimuli may further increase transcriptional activity. Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate but short-lived, while FRA1 and FRA2 expression persists longer. FRA1 is involved in cell motility, invasiveness, and inhibits apoptosis. Elevated in many cancers and associated with tumorigenesis and cancer progression. Involved in Erk2-mediated Epithelial-to-Mesenchymal Transition (EMT) pathway. ERK2/FRA2 regulate ZEB1/2 expression, known to be associated with the EMT. Smad2/3-Fra1 complexes may reflect activation of the Smad/AP-1-dependent TGF¿-induced breast cancer invasion program. Activation of FRA1/C-JUN by ERK/AKT pathways can induce EZH2 overexpression, silencing integrin alpha-2 expression, and increasing the metastatic potential of colorectal cancer. Belongs to the bZIP family. Fos subfamily.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; presynaptic membrane; neuron projection; nucleus; cytosol

Molecular Function: protein binding; transcription factor activity

Biological Process: response to drug; transcription from RNA polymerase II promoter; response to gravity; response to cAMP; in utero embryonic development; positive regulation of apoptosis; response to virus; vitellogenesis; positive regulation of cell cycle; female pregnancy; learning; chemotaxis; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; response to hydrogen peroxide; response to mechanical stimulus; response to cytokine stimulus; positive regulation of cell proliferation; cellular response to extracellular stimulus; cellular defense response; positive regulation of transcription factor activity; response to progesterone stimulus; response to corticosterone stimulus