TCPTP Active Protein | PTPN2 active protein

TCPTP Protein, Mouse, Recombinant (aa 2-314, His Tag)

Cat. Num. AAA8122987

• Gene Names: Ptpn2; Ptpt; TC-PTP; AI325124
• Purity: >90% as determined by SDS-PAGE
• Synonyms: TCPTP; TCPTP Protein; Mouse; Recombinant (aa 2-314; His Tag); Mouse PTPN2/TC-PTP Protein (aa 2-314; AI325124 Protein; Ptpt Protein; TC-PTP Protein; protein tyrosine phosphatase; non-receptor type 2; PTPN2 active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH8.0, 20% gly, 0.1mM TCEP. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ser2-Asn314

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mouse PTPN2 (Q06180-1)(Ser 2-Asn 314) was fused with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor 988-998 phosphopeptide substrate The specific activity is >15nMoles/min/ug.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PTPN2 active protein

Background: Tyrosine-protein phosphatase non-receptor type 2, also known as T-cell protein-tyrosine phosphatase, PTPN2 and PTPT, is a cytoplasm protein that belongs to the protein-tyrosine phosphatase family and Non-receptor class 1 subfamily. Members of the protein tyrosine phosphatase (PTP) family share a highly conserved catalytic motif, which is essential for the catalytic activity. TC-PTP/PTPN2 is a cytosolic tyrosine phosphatase that functions as a negative regulator of a variety of tyrosine kinases and other signaling proteins. The expression of TC-PTP/PTPN2 plays a role of tumor suppressor and may modulate response to treatment. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. TC-PTP/PTPN2 is an enzyme that is essential for the proper functioning of the immune system and that participates in the control of cell proliferation, and inflammation. TC-PTP/PTPN2 was identified as a negative regulator of NUP214-ABL1 kinase activity.

Product Categories/Family for PTPN2 active protein

Phosphatases and Regulators

References

Cool D., et al.,(1989), cDNA isolated from a human T-cell library encodes a member of the protein-tyrosine-phosphatase family. Proc. Natl. Acad. Sci. U.S.A. 86:5257-5261.Ota T., et al., (2004), Complete sequencing and characterization of 21,243 full-length human cDNAs.Nat. Genet. 36:40-45.Nusbaum C., et al.,(2005), DNA sequence and analysis of human chromosome 18.Nature 437:551-555.

NCBI and Uniprot Product Information

• NCBI GI #: 187608416
• NCBI GeneID: 19255
• NCBI Accession #: NP_001120649.1
• NCBI GenBank Nucleotide #: NM_001127177.1
• UniProt Accession #: Q06180; Q3V259; Q922E7
• Molecular Weight: 44,573 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 2 isoform b
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 2
• NCBI Official Symbol: Ptpn2
• NCBI Official Synonym Symbols: Ptpt; TC-PTP; AI325124
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 2
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 2
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: Ptpn2
• UniProt Synonym Gene Names: Ptpt; Protein-tyrosine phosphatase PTP-2

Uniprot Description

PTPN2: a type 2 non-receptor phospho-tyrosine-protein phosphatase (PTP). Two alternatively spliced isoforms are differenyially expressed. Isoform A is a major PTP expressed in human tissues, while isoform B is expressed mainly in T-cells and in placenta.

Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 18|18 E1

Cellular Component: endoplasmic reticulum; ER-Golgi intermediate compartment; nucleus; plasma membrane

Molecular Function: integrin binding; non-membrane spanning protein tyrosine phosphatase activity; protein binding; protein kinase binding; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; syntaxin binding

Biological Process: B cell differentiation; erythrocyte differentiation; glucose homeostasis; insulin receptor signaling pathway; negative regulation of cell proliferation; negative regulation of chemotaxis; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of inflammatory response; negative regulation of insulin receptor signaling pathway; negative regulation of macrophage differentiation; negative regulation of T cell receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of tyrosine phosphorylation of Stat1 protein; negative regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of tyrosine phosphorylation of Stat6 protein; positive regulation of gluconeogenesis; protein amino acid dephosphorylation; T cell differentiation