SDS-Page
(Recombinant PTPN2 protein gel. Recombinant PTPN2 protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW: 75.7 kDa Purity: > 50%)
PTPN2 recombinant protein
Recombinant PTPN2 protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant PTPN2 protein gel. Recombinant PTPN2 protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW: 75.7 kDa Purity: > 50%)
SDS-Page
(Recombinant PTPN2 protein gel. Recombinant PTPN2 protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. MW: 75.7 kDa Purity: > 50%)
Testing Data
(Recombinant PTPN2 protein activity assay. 1 mM pNPP was incubated with different concentrations of PTPN2 protein ina 100 ul reaction with buffer including 50 mM HEPES pH 7.5, 2 mM EDTA, 3 mM DTT, 100 mM NaCl at RT. The absorbance at 405 nm was continuously monitored for 30 minutes.)
Testing Data
(Recombinant PTPN2 protein activity assay. 1 mM pNPP was incubated with different concentrations of PTPN2 protein ina 100 ul reaction with buffer including 50 mM HEPES pH 7.5, 2 mM EDTA, 3 mM DTT, 100 mM NaCl at RT. The absorbance at 405 nm was continuously monitored for 30 minutes.)
Background: PTPN2 (Protein Tyrosine Phosphatase, Non-Receptor Type 2, also known as TCELLPTP, TCPTP, TC- PTP, PTPT, PTN2) is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family have a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation as signaling molecules. Epidermal growth factor receptor (EGFR) and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. PTPN2 dephosphorylates multiple substrates including receptor protein tyrosine kinases INSR, EGFR, CSF1R, PDGFR and non-receptor protein tyrosine kinases like JAK1, JAK2, JAK3, Src family kinases, STAT1, STAT3, STAT5A, STAT5B and STAT6 either in the nucleus or the cytoplasm.
NCBI and Uniprot Product Information
Uniprot Description
PTPN2: a type 2 non-receptor phospho-tyrosine-protein phosphatase (PTP). Two alternatively spliced isoforms are differenyially expressed. Isoform A is a major PTP expressed in human tissues, while isoform B is expressed mainly in T-cells and in placenta.
Protein type: Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.48; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 18p11.3-p11.2
Cellular Component: nucleoplasm; endoplasmic reticulum; ER-Golgi intermediate compartment; plasma membrane; nucleus
Molecular Function: integrin binding; protein binding; syntaxin binding; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; protein kinase binding
Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat1 protein; cytokine and chemokine mediated signaling pathway; negative regulation of insulin receptor signaling pathway; negative regulation of T cell receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of chemotaxis; glucose homeostasis; negative regulation of cell proliferation; positive regulation of gluconeogenesis; negative regulation of macrophage differentiation; negative regulation of inflammatory response; B cell differentiation; negative regulation of tyrosine phosphorylation of Stat3 protein; insulin receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat6 protein; erythrocyte differentiation; T cell differentiation
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Product Notes
The PTPN2 ptpn2 (Catalog #AAA388294) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PTPN2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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