PTPN2 / PTPT recombinant protein

Recombinant Human PTPN2 / PTPT Protein

Cat. Num. AAA2546173

• Gene Names: PTPN2; PTN2; PTPT; TCPTP; TC-PTP; TCELLPTP
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: PTPN2 / PTPT; Recombinant Human PTPN2 / PTPT Protein; PTPN2; PTN2; PTPT; TC-PTP; TCELLPTP; TCPTP; PTPN2 / PTPT recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0
• Sequence Length: 410

• Application Notes: The secreted recombinant human PTPN2 consists of 316 amino acids and predicts a molecular mass of 36.8 KDa. The apparent molecular mass of the protein is approximately 37 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Gly
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for PTPN2 / PTPT recombinant protein

Background: Tyrosine-protein phosphatase non-receptor type 2, also known as T-cell protein-tyrosine phosphatase, PTPN2 and PTPT, is a cytoplasm protein which belongs to the protein-tyrosine phosphatase family and Non-receptor class 1 subfamily. Members of the protein tyrosine phosphatase (PTP) family share a highly conserved catalytic motif, which is essential for the catalytic activity. TC-PTP / PTPN2 is a cytosolic tyrosine phosphatase that functions as a negative regulator of a variety of tyrosine kinases and other signaling proteins. The expression of TC-PTP / PTPN2 plays a role of tumor suppressor and may modulate response to treatment. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. TC-PTP / PTPN2 is an enzyme that is essential for the proper functioning of the immune system and that participates in the control of cell proliferation, and inflammation. TC-PTP / PTPN2 was identified as a negative regulator of NUP214-ABL1 kinase activity.

Description: A DNA sequence encoding the human PTPN2 ( P17706-1) (Met 1-Asn 314) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 333108235
• NCBI GeneID: 5771
• NCBI Accession #: NP_001193942.1
• NCBI GenBank Nucleotide #: NM_001207013.1
• UniProt Accession #: P17706; Q96AU5; Q96HR2; A8K955; A8MXU3; K7ENG3
• Molecular Weight: 48,017 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 2 isoform 4
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 2
• NCBI Official Symbol: PTPN2
• NCBI Official Synonym Symbols: PTN2; PTPT; TCPTP; TC-PTP; TCELLPTP
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 2
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 2
• UniProt Gene Name: PTPN2
• UniProt Synonym Gene Names: PTPT; TCPTP

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Two highly related but distinctly processed pseudogenes that localize to chromosomes 1 and 13, respectively, have been reported. [provided by RefSeq, May 2011]

Uniprot Description

PTPN2: a type 2 non-receptor phospho-tyrosine-protein phosphatase (PTP). Two alternatively spliced isoforms are differenyially expressed. Isoform A is a major PTP expressed in human tissues, while isoform B is expressed mainly in T-cells and in placenta.

Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 18p11.21

Cellular Component: endoplasmic reticulum; ER-Golgi intermediate compartment; nucleoplasm; plasma membrane

Molecular Function: integrin binding; protein binding; protein kinase binding; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; syntaxin binding

Biological Process: B cell differentiation; erythrocyte differentiation; glucose homeostasis; insulin receptor signaling pathway; negative regulation of cell proliferation; negative regulation of chemotaxis; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of inflammatory response; negative regulation of insulin receptor signaling pathway; negative regulation of macrophage differentiation; negative regulation of T cell receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat1 protein; negative regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of gluconeogenesis; T cell differentiation