Rabbit anti-Human Recoverin Polyclonal Antibody | anti-RCVRN antibody

Recoverin Antibody (C-term)

Cat. Num. AAA9213234

• Gene Names: RCVRN; RCV1
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Recoverin; Polyclonal Antibody; Recoverin Antibody (C-term); Cancer-associated retinopathy protein; Protein CAR; RCVRN; RCV1; anti-RCVRN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Recoverin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-200 amino acids from the C-terminal region of human Recoverin.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 169-200
• Sequence Length: 200

• Applicable Applications for anti-RCVRN antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(The anti-Recoverin C-term Pab is used in Western blot to detect Recoverin in Y79 cell lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-RCVRN antibody

Recoverin belongs to a high-affinity calcium-binding family that includes neuronal calcium sensor-1, visinin-like proteins (VILIPs), guanylate cyclase-activating proteins (GCAPs), and Kv-channel interacting proteins (KchIPs). Features common to this family include four calcium-binding EF-hand domains, and an N-terminal myristoylation sequence. This family of proteins has been implicated in a broad range of cellular signaling functions, including phototransduction and neurotransmitter release, lipid metabolism, gene expression, and ion channel regulation. Myristoylation, the post-translational addition of a fatty acid tail, has been shown to have functional significance for other calcium-binding protein family members. Recoverin is subject to the posttranslational modification of myristoylation. Binding of calcium to recoverin elicits a change in conformation that exposes the buried hydrophobic myristoyl moiety to interaction with cell membranes and other cellular proteins.

Product Categories/Family for anti-RCVRN antibody

Neuroscience; Signal Transduction

References

Wiechmann, A., et al., Curr. Eye Res. 26(1):25-32 (2003). Matsubara, S., et al., Br. J. Cancer 74(9):1419-1422 (1996). Yamaji, Y., et al., Int. J. Cancer 65(5):671-676 (1996). McGinnis, J.F., et al., Mamm. Genome 4(1):43-45 (1993). Wiechmann, A.F., et al., Exp. Eye Res. 56(4):463-470 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 4506459
• NCBI GeneID: 5957
• NCBI Accession #: NP_002894.1
• NCBI GenBank Nucleotide #: NM_002903.2
• UniProt Accession #: P35243; Q53XL0
• Molecular Weight: 23130
• NCBI Official Full Name: recoverin
• NCBI Official Synonym Full Names: recoverin
• NCBI Official Symbol: RCVRN
• NCBI Official Synonym Symbols: RCV1
• NCBI Protein Information: recoverin
• UniProt Protein Name: Recoverin
• Protein Family: Recoverin
• UniProt Gene Name: RCVRN
• UniProt Synonym Gene Names: RCV1; Protein CAR
• UniProt Entry Name: RECO_HUMAN

NCBI Description

This gene encodes a member of the recoverin family of neuronal calcium sensors. The encoded protein contains three calcium-binding EF-hand domains and may prolong the termination of the phototransduction cascade in the retina by blocking the phosphorylation of photo-activated rhodopsin. Recoverin may be the antigen responsible for cancer-associated retinopathy. [provided by RefSeq, Jul 2008]

Uniprot Description

RCV1: Seems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin. May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent manner. The calcium-bound recoverin prolongs the photoresponse. Belongs to the recoverin family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: dendrite

Molecular Function: calcium sensitive guanylate cyclase activator activity; calcium ion binding

Biological Process: rhodopsin mediated signaling; phototransduction, visible light; regulation of rhodopsin mediated signaling; visual perception; positive regulation of guanylate cyclase activity; regulation of calcium ion transport; signal transduction