TCPTP Active Protein | PTPN2 active protein

TCPTP Protein, Human, Recombinant (aa 1-314, His & GST Tag)

Cat. Num. AAA8120363

• Gene Names: PTPN2; PTN2; PTPT; TCPTP; TC-PTP; TCELLPTP
• Purity: >75% as determined by SDS-PAGE
• Synonyms: TCPTP; TCPTP Protein; Human; Recombinant (aa 1-314; His & GST Tag); Human PTPN2/TC-PTP Protein (aa 1-314; PTN2 Protein; PTPT Protein; TC-PTP Protein; TCELLPTP Protein; protein tyrosine phosphatase; non-receptor type 2; PTPN2 active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >75% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 300mM NaCl, 10% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn314

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the amino acid (Met 1-Asn 314) of human PTPN2 (P17706-1) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor 988-998 phosphopeptide substrate. The specific activity is >20nMoles/min/ug.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for PTPN2 active protein

Background: Tyrosine-protein phosphatase non-receptor type 2, also known as T-cell protein-tyrosine phosphatase, PTPN2 and PTPT, is a cytoplasm protein that belongs to the protein-tyrosine phosphatase family and Non-receptor class 1 subfamily. Members of the protein tyrosine phosphatase (PTP) family share a highly conserved catalytic motif, which is essential for the catalytic activity. TC-PTP/PTPN2 is a cytosolic tyrosine phosphatase that functions as a negative regulator of a variety of tyrosine kinases and other signaling proteins. The expression of TC-PTP/PTPN2 plays a role of tumor suppressor and may modulate response to treatment. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. TC-PTP/PTPN2 is an enzyme that is essential for the proper functioning of the immune system and that participates in the control of cell proliferation, and inflammation. TC-PTP/PTPN2 was identified as a negative regulator of NUP214-ABL1 kinase activity.

Product Categories/Family for PTPN2 active protein

Phosphatases and Regulators

References

Cool D., et al.,(1989), cDNA isolated from a human T-cell library encodes a member of the protein-tyrosine-phosphatase family. Proc. Natl. Acad. Sci. U.S.A. 86:5257-5261.Ota T., et al., (2004), Complete sequencing and characterization of 21,243 full-length human cDNAs.Nat. Genet. 36:40-45.Nusbaum C., et al.,(2005), DNA sequence and analysis of human chromosome 18.Nature 437:551-555.

NCBI and Uniprot Product Information

• NCBI GI #: 229462762
• NCBI GeneID: 5771
• UniProt Accession #: P17706; Q96AU5; Q96HR2; A8K955; A8MXU3; K7ENG3
• Molecular Weight: 415
• NCBI Official Full Name: Tyrosine-protein phosphatase non-receptor type 2
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 2
• NCBI Official Symbol: PTPN2
• NCBI Official Synonym Symbols: PTN2; PTPT; TCPTP; TC-PTP; TCELLPTP
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 2; T-cell protein tyrosine phosphatase
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 2
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: PTPN2
• UniProt Synonym Gene Names: PTPT; TCPTP
• UniProt Entry Name: PTN2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Two highly related but distinctly processed pseudogenes that localize to chromosomes 1 and 13, respectively, have been reported. [provided by RefSeq, May 2011]

Uniprot Description

PTPN2: a type 2 non-receptor phospho-tyrosine-protein phosphatase (PTP). Two alternatively spliced isoforms are differenyially expressed. Isoform A is a major PTP expressed in human tissues, while isoform B is expressed mainly in T-cells and in placenta.

Protein type: Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.48; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 18p11.3-p11.2

Cellular Component: nucleoplasm; endoplasmic reticulum; ER-Golgi intermediate compartment; plasma membrane; nucleus

Molecular Function: integrin binding; protein binding; syntaxin binding; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; protein kinase binding

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat1 protein; cytokine and chemokine mediated signaling pathway; negative regulation of insulin receptor signaling pathway; negative regulation of T cell receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of chemotaxis; glucose homeostasis; negative regulation of cell proliferation; positive regulation of gluconeogenesis; negative regulation of macrophage differentiation; negative regulation of inflammatory response; B cell differentiation; negative regulation of tyrosine phosphorylation of Stat3 protein; insulin receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat6 protein; erythrocyte differentiation; T cell differentiation