Principle of the Assay: Uricase catalyzes the decomposition of uric acid into allantoin, CO2 and H2O2. Under the action of peroxidase, H2O2 oxidizes the non-fluorescent probe into the fluorescent substance. By measuring the fluorescence value of the system, the corresponding uric acid content can be calculated.
Background/Introduction: Uric acid, a purine metabolite, is degraded into allantoin by uricase in most mammals. Due to the absence of urate oxidase gene, uric acid is the final product of purine metabolism in humans, so the level of uric acid in human blood is higher than that in most mammals. Uric acid is a physiologically important plasma antioxidant that effectively protects biological targets from the oxidation of hydroxyl radicals, hypochloric acid and peroxynitrite.
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Product Notes
The UA (Catalog #AAA2567664) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Uric Acid (UA), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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