Typical Testing Data/Standard Curve (for reference only)
Catalase (CAT) Assay Kit | CAT assay kit
Catalase (CAT) Activity Fluorometric Assay Kit
Synonyms
Catalase (CAT); Catalase (CAT) Activity Fluorometric Assay Kit; CAT assay kit
Samples
Serum, plasma, and tissue samples
Detection Range
0.01-6.51 U/L
Sensitivity
0.01 U/L
Preparation and Storage
Store at -20 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Testing Data
Testing Data
Related Product Information for CAT assay kit
Intended Uses: This kit can be used to measure Catalase (CAT) activity in serum, plasma, and tissue samples.
Principle of the Assay: Catalase can decompose H2O2 to generate H2O and O2, the residual H2O2 in the detection system react with the fluorescent substance, and the content of residual H2O2 is proportional to the fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 587 nm, the catalase activity is inversely proportional to the fluorescence intensity.
Background/Introduction: CAT is an enzyme in organism that can efficiently and specifically decompose hydrogen peroxide and is a binding enzyme with iron porphyrin as an auxiliary group. CAT clears hydrogen peroxide in the body and protects cells from the toxicity of H2O2. CAT can also oxidize certain cytotoxic substances, such as formaldehyde, formic acid, phenol and ethanol. According to the difference of catalytic center structure, CAT can be divided into two types, one is iron porphyrin structure, also known as iron porphyrin enzyme, the other contain manganese ion, also known as manganese catalase. CAT is common in breathing organisms. It is mainly found in chloroplasts, mitochondria, endoplasmic reticulum, liver and red blood cells of animals.
Principle of the Assay: Catalase can decompose H2O2 to generate H2O and O2, the residual H2O2 in the detection system react with the fluorescent substance, and the content of residual H2O2 is proportional to the fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 587 nm, the catalase activity is inversely proportional to the fluorescence intensity.
Background/Introduction: CAT is an enzyme in organism that can efficiently and specifically decompose hydrogen peroxide and is a binding enzyme with iron porphyrin as an auxiliary group. CAT clears hydrogen peroxide in the body and protects cells from the toxicity of H2O2. CAT can also oxidize certain cytotoxic substances, such as formaldehyde, formic acid, phenol and ethanol. According to the difference of catalytic center structure, CAT can be divided into two types, one is iron porphyrin structure, also known as iron porphyrin enzyme, the other contain manganese ion, also known as manganese catalase. CAT is common in breathing organisms. It is mainly found in chloroplasts, mitochondria, endoplasmic reticulum, liver and red blood cells of animals.
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Product Notes
The CAT (Catalog #AAA2567662) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Catalase (CAT), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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