Western Blot (WB)
(Rad17 phospho Ser647 pAb tested by Western blot. Rad17 detection by Western blot. The analysis of phospho-RAD17 was performed using the Rad17 phospho Ser647 pAb and partial recombinant Rad17 protein containing phospho Ser647 (lane A) and the same sequence without a phospho serine (lane B).)
Mouse anti-Human, Rat Rad17 phospho Ser647 Polyclonal Antibody | anti-Rad17 antibody
Rad17 phospho Ser647 antibody (pAb)
Reactivity
Human, Rat
Applications
Western Blot
Purity
Affinity Purified
Synonyms
Rad17 phospho Ser647; Polyclonal Antibody; Rad17 phospho Ser647 antibody (pAb); CCYC; R24L; RAD24; HRAD17; RAD17SP; anti-Rad17 antibody
Host
Mouse
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Concentration
0.5ug/ul (varies by lot)
Applicable Applications for anti-Rad17 antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 0.1-1ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
WB: 0.1-1ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
Conjugation
Phosphorylated
Immunogen
This Rad17 phospho Ser647 antibody was raised against a synthetic peptide corresponding to mouse Rad17 phosphorylated at serine 647.
Abs Residue
Ser
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(Rad17 phospho Ser647 pAb tested by Western blot. Rad17 detection by Western blot. The analysis of phospho-RAD17 was performed using the Rad17 phospho Ser647 pAb and partial recombinant Rad17 protein containing phospho Ser647 (lane A) and the same sequence without a phospho serine (lane B).)
Western Blot (WB)
(Rad17 phospho Ser647 pAb tested by Western blot. Rad17 detection by Western blot. The analysis of phospho-RAD17 was performed using the Rad17 phospho Ser647 pAb and partial recombinant Rad17 protein containing phospho Ser647 (lane A) and the same sequence without a phospho serine (lane B).)
Related Product Information for anti-Rad17 antibody
Short Description: Our Rad17 phospho Ser647 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human and Rat samples.
Background: Rad17 Ser647 (Rad24, CCYC, R24L) is essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Rad17 has a weak ATPase activity required for binding to chromatin. Rad17 participates in the recruitment of the Rad1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. Rad17 may also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
Background: Rad17 Ser647 (Rad24, CCYC, R24L) is essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Rad17 has a weak ATPase activity required for binding to chromatin. Rad17 participates in the recruitment of the Rad1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. Rad17 may also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
Product Categories/Family for anti-Rad17 antibody
NCBI and Uniprot Product Information
Molecular Weight
77kDa
Protein Family
Similar Products
Product Notes
The Rad17 (Catalog #AAA388418) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Rad17 phospho Ser647 antibody (pAb) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Rad17 phospho Ser647 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 0.1-1ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Researchers should empirically determine the suitability of the Rad17 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rad17 phospho Ser647, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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