Typical Testing Data/Standard Curve (for reference only)
Free Fatty Acids (FFA) Assay Kit | FFA assay kit
Free Fatty Acids (FFA) Fluorometric Assay Kit
Synonyms
Free Fatty Acids (FFA); Free Fatty Acids (FFA) Fluorometric Assay Kit; FFA assay kit
Samples
Serum, plasma, animal tissue samples
Detection Range
0.58-20 umol/L
Sensitivity
0.58 umol/L
Preparation and Storage
Store at -20 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Testing Data
Testing Data
Related Product Information for FFA assay kit
Intended Uses: This kit can be used to measure the non-esterified free fatty acids (FFA) content in serum, plasma, animal tissue samples.
Principle of the Assay: Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen peroxide in the presence of acyl oxidase. In the presence of the enzyme and probe, hydrogen peroxide react to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is directly proportional to the concentration of free fatty acids.
Background/Introduction: Free fatty acids, also known as non-esterified fatty acids, are derived from dietary or the metabolism of adipose tissue. In adipose tissue, hormonesensitive lipase (HSL) decomposes triglycerides to produce glyc erol and fatty acids. Circulating in the body with free fatty acids combined with plasma albumin, used as an energy source easily absorbed by muscles, brains, and other tissues and organs. FFA is not only the product of fat hydrolysis, but also the substrate of fat synthesis. The concentration of FFA is related to lipid metabolism, glucose metabolism and endocrine function.
Principle of the Assay: Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen peroxide in the presence of acyl oxidase. In the presence of the enzyme and probe, hydrogen peroxide react to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is directly proportional to the concentration of free fatty acids.
Background/Introduction: Free fatty acids, also known as non-esterified fatty acids, are derived from dietary or the metabolism of adipose tissue. In adipose tissue, hormonesensitive lipase (HSL) decomposes triglycerides to produce glyc erol and fatty acids. Circulating in the body with free fatty acids combined with plasma albumin, used as an energy source easily absorbed by muscles, brains, and other tissues and organs. FFA is not only the product of fat hydrolysis, but also the substrate of fat synthesis. The concentration of FFA is related to lipid metabolism, glucose metabolism and endocrine function.
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Product Notes
The FFA (Catalog #AAA2567669) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Free Fatty Acids (FFA), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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