Typical Testing Data/Standard Curve (for reference only)
Total Cholesterol and Cholesteryl Ester Assay Kit
Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit
Synonyms
Total Cholesterol and Cholesteryl Ester; Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit; Total Cholesterol and Cholesteryl Ester assay kit
Samples
Serum, plasma, animal tissue and cell samples
Detection Range
0.12-30 umol/L
Sensitivity
0.12 umol/L
Preparation and Storage
Store at -20 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for Total Cholesterol and Cholesteryl Ester assay kit
Intended Uses: This kit can be used for determination of Total Cholesterol (TC) and Cholesterol Esters (CE) content in serum, plasma, animal tissue and cell samples.
Principle of the Assay: Total Cholesterol (TC) includes free cholesterol (FC) and cholesteryl esters (CE). Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce delta4-cholestenone and hydrogen peroxide. In the presence of the enzyme and probe, hydrogen peroxide can be catalyzed to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is proportional to the cholesterol concentration.
Background/Introduction: Cholesterol is a kind of sterol and lipid in cell membrane. Most of cholesterol in blood exists in the form of cholesterol ester. Lecithin-cholesterol acyltransferase in human plasma is an enzyme that catalyzes the formation of cholesterol ester. Cholesterol synthesized or deposited in peripheral cells returns to the liver through the reverse cholesterol transport system for reusing or regaining bile acids.
Principle of the Assay: Total Cholesterol (TC) includes free cholesterol (FC) and cholesteryl esters (CE). Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce delta4-cholestenone and hydrogen peroxide. In the presence of the enzyme and probe, hydrogen peroxide can be catalyzed to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is proportional to the cholesterol concentration.
Background/Introduction: Cholesterol is a kind of sterol and lipid in cell membrane. Most of cholesterol in blood exists in the form of cholesterol ester. Lecithin-cholesterol acyltransferase in human plasma is an enzyme that catalyzes the formation of cholesterol ester. Cholesterol synthesized or deposited in peripheral cells returns to the liver through the reverse cholesterol transport system for reusing or regaining bile acids.
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Product Notes
The Total Cholesterol and Cholesteryl Ester (Catalog #AAA2567666) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Total Cholesterol and Cholesteryl Ester, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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