Typical Testing Data/Standard Curve (for reference only)
Total Sulfhydryl Group/Total Thiol Assay Kit
Total Sulfhydryl Group/Total Thiol (-SH) Colorimetric Assay Kit
Synonyms
Total Sulfhydryl Group/Total Thiol; Total Sulfhydryl Group/Total Thiol (-SH) Colorimetric Assay Kit; Total Sulfhydryl Group/Total Thiol assay kit
Samples
Serum, plasma, animal tissue samples
Detection Range
9.91-1000 umol/L
Sensitivity
9.91 umol/L
Preparation and Storage
Store at 2-8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Testing Data
Testing Data
Related Product Information for Total Sulfhydryl Group/Total Thiol assay kit
Intended Uses: This kit can measure total (-SH) content in serum, plasma, animal tissue samples.
Principle of the Assay: Sulfhydryl compounds react with 5,5' -dithiobis (2-nitrobenzoic acid) under neutral or alkaline conditions to produce a yellow product which have a maximum absorption peak at 412 nm. Measure the OD value and calculate the total mercapto content indirectly.
Background/Introduction: Total sulfhydryl group/total thiol (-SH) is an important protein structure and REDOX reactive group in organisms. Sulfhydryl is one of the most active and ubiquitous ligands in biological systems. It is found in most proteins, but also in some low molecular weight substances. Sulfhydryl groups play an important role in biochemical processes, not only for the REDOX mechanism, but also for the enhancement of the function of some hydrolyzed biocatalysts. Compounds containing sulfhydryl groups are called thiols. Decreased thiol levels are found in various diseases.
Principle of the Assay: Sulfhydryl compounds react with 5,5' -dithiobis (2-nitrobenzoic acid) under neutral or alkaline conditions to produce a yellow product which have a maximum absorption peak at 412 nm. Measure the OD value and calculate the total mercapto content indirectly.
Background/Introduction: Total sulfhydryl group/total thiol (-SH) is an important protein structure and REDOX reactive group in organisms. Sulfhydryl is one of the most active and ubiquitous ligands in biological systems. It is found in most proteins, but also in some low molecular weight substances. Sulfhydryl groups play an important role in biochemical processes, not only for the REDOX mechanism, but also for the enhancement of the function of some hydrolyzed biocatalysts. Compounds containing sulfhydryl groups are called thiols. Decreased thiol levels are found in various diseases.
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Product Notes
The Total Sulfhydryl Group/Total Thiol (Catalog #AAA2567691) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Total Sulfhydryl Group/Total Thiol, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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