Typical Testing Data/Standard Curve (for reference only)
Total Carbohydrate Assay Kit
Total Carbohydrate Assay Kit
Synonyms
Total Carbohydrate; Total Carbohydrate Assay Kit; Total Carbohydrate assay kit
Preparation and Storage
Store glucose standard at 4 degree C and store the other components at room temperature.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Total Carbohydrate assay kit
Principle of the Assay: Total Carbohydrate Assay Kit measures total carbohydrate within food samples, urine, serum, plasma, lysate, or tissue samples based on the phenol-sulfuric acid method. Carbohydrates are hydrolyzed to furfural and other derivative forms in the presence of sulfuric acid. Upon addition of Developing Solution, a chromagen is formed that can be detected at 490 nm (Figure 1).
Background/Introduction: Carbohydrates are molecules made up of oxygen, hydrogen, and carbon. Typically carbohydrates contain a hydrogen:oxygen atom ratio of 2:1 (as with water). In the field of biochemistry, carbohydrates are viewed synonymously with saccharides, which describe sugars, starch, and cellulose. The saccharides can be further categorized as monosaccharides, disaccharides, oligosaccharides, and polysaccharides. Typically the lower molecular weight monosaccharides (such as glucose) and disaccharides (such as sucrose) are referred to as sugars. Carbohydrates perform key roles in living organisms such as storing energy, as parts of coenzymes, as part of genetic material (DNA and RNA) as well as structural roles. The measurement of total carbohydrate concentration is important in several fields such as the food industry, pharmaceutical research, petroleum industry, as well as environmental research. Many techniques such as light scattering, nuclear magnetic resonance, capillary electrophoresis, infrared spectroscopy, and chromatography have been used to detect total carbohydrates, but these techniques are costly, time consuming, and require complex analytical skills. Total Carbohydrate Assay Kit is a simple colorimetric assay that measures the amount of total carbohydrate present in foods, urine, plasma, serum, tissue homogenates, or cell lysates in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, standards and unknown samples. Sample carbohydrate concentrations are determined by comparison with a known glucose standard. The kit has a detection sensitivity limit of 62.5 uM glucose.
Background/Introduction: Carbohydrates are molecules made up of oxygen, hydrogen, and carbon. Typically carbohydrates contain a hydrogen:oxygen atom ratio of 2:1 (as with water). In the field of biochemistry, carbohydrates are viewed synonymously with saccharides, which describe sugars, starch, and cellulose. The saccharides can be further categorized as monosaccharides, disaccharides, oligosaccharides, and polysaccharides. Typically the lower molecular weight monosaccharides (such as glucose) and disaccharides (such as sucrose) are referred to as sugars. Carbohydrates perform key roles in living organisms such as storing energy, as parts of coenzymes, as part of genetic material (DNA and RNA) as well as structural roles. The measurement of total carbohydrate concentration is important in several fields such as the food industry, pharmaceutical research, petroleum industry, as well as environmental research. Many techniques such as light scattering, nuclear magnetic resonance, capillary electrophoresis, infrared spectroscopy, and chromatography have been used to detect total carbohydrates, but these techniques are costly, time consuming, and require complex analytical skills. Total Carbohydrate Assay Kit is a simple colorimetric assay that measures the amount of total carbohydrate present in foods, urine, plasma, serum, tissue homogenates, or cell lysates in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, standards and unknown samples. Sample carbohydrate concentrations are determined by comparison with a known glucose standard. The kit has a detection sensitivity limit of 62.5 uM glucose.
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Product Notes
The Total Carbohydrate (Catalog #AAA169243) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Total Carbohydrate, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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