Rabbit anti-Human BCAT2 Polyclonal Antibody | anti-BCAT2 antibody

BCAT2 (Branched Chain Aminotransferase 2, Mitochondrial, BCAM, BCT2) (FITC)

Cat. Num. AAA6371082

• Gene Names: BCAT2; BCAM; BCT2; HVLI; PP18; BCATM
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BCAT2; Polyclonal Antibody; BCAT2 (Branched Chain Aminotransferase 2; Mitochondrial; BCAM; BCT2) (FITC); BCATM; BCT2; ECA40; Branched-chain-amino-acid aminotransferase; mitochondrial; Placental protein 18; anti-BCAT2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BCAT2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 392

• Applicable Applications for anti-BCAT2 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human BCAT2, aa1-392 (NP_001181.2).
• Immunogen Sequence: MAAAALGQIWARKLLSVPWLLCGPRRYASSSFKAADLQLEMTQKPHKKPGPGEPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of BCAT2 expression in transfected 293T cell line by BCAT2 polyclonal antibody. Lane 1: BCAT2 transfected lysate (44.3kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-BCAT2 antibody

BCAT2 is a branched chain aminotransferase found in mitochondria. This protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine.

Product Categories/Family for anti-BCAT2 antibody

Antibodies; Enzymes; Aminotransferase

NCBI and Uniprot Product Information

• NCBI GI #: 50658084
• NCBI GeneID: 587
• NCBI Accession #: NP_001181.2
• NCBI GenBank Nucleotide #: NM_001190.2
• UniProt Accession #: O15382
• NCBI Official Full Name: branched-chain-amino-acid aminotransferase, mitochondrial isoform a
• NCBI Official Synonym Full Names: branched chain amino acid transaminase 2
• NCBI Official Symbol: BCAT2
• NCBI Official Synonym Symbols: BCAM; BCT2; HVLI; PP18; BCATM
• NCBI Protein Information: branched-chain-amino-acid aminotransferase, mitochondrial
• UniProt Protein Name: Branched-chain-amino-acid aminotransferase, mitochondrial
• UniProt Gene Name: BCAT2
• UniProt Synonym Gene Names: BCATM; BCT2; ECA40; BCAT(m); PP18
• UniProt Entry Name: BCAT2_HUMAN

NCBI Description

This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

BCAT2: Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids. Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. Homodimer. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; EC 2.6.1.42; Transferase; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Mitochondrial

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: branched-chain-amino-acid transaminase activity

Biological Process: valine metabolic process; branched chain family amino acid catabolic process; leucine metabolic process; isoleucine catabolic process; branched chain family amino acid biosynthetic process