Typical Testing Data/Standard Curve (for reference only)
Total Bile Acid Assay Kit
Total Bile Acid Assay Kit
Synonyms
Total Bile Acid; Total Bile Acid Assay Kit; Total Bile Acid assay kit
Preparation and Storage
Upon receipt, store the kit at -80 degree C. If the kit will be used in multiple experiments, aliquot each component before freezing to avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Total Bile Acid assay kit
Principle of the Assay: The Total Bile Acid Assay Kit measures the total bile acid within serum, plasma, and cell or tissue lysate samples. The assay is based on an enzyme driven reaction: when bile acids are incubated in the presence of 3alpha-hydroxysteroid dehydrogenase (3alpha-HSD), NADH, and thio-NAD+, thio-NAD+ is converted to its reduced form Thio-NADH. Thio-NADH is then detected colorimetrically as an absorbance increase at 405 nm (Figure 1).
Background/Introduction: Bile is a complex mixture of lipids, protein, carbohydrates, mineral salts, vitamins, and various trace elements, with bile acids making up about 67% of the total composition. Bile acids are produced from excess cholesterol, secreted from the liver, absorbed into the small intestines, and returned to the liver with portal blood. While bile acid synthesis is critical for the removal of cholesterol from the body, bile acids are also needed for proper uptake of dietary lipids, fat soluble vitamins, and other nutrients into the small intestines. Under physiological conditions, newly synthesized bile acids are conjugated to glycine or taurine to form bile salts, and not much free bile acid is actually found in bile. Determining circulatory levels of bile acids can be used to identify or identify certain liver diseases. In addition, elevated serum bile levels have been observed in intrahepatic cholestasis of pregnancy cases. The Total Bile Acid Assay Kit is a simple colorimetric assay that measures the amount of total bile acid present in plasma, serum, tissue homogenates, or cell lysates in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, bile acid standards and unknown samples. Sample bile acid concentrations are determined by comparison with a known bile acid standard.
Background/Introduction: Bile is a complex mixture of lipids, protein, carbohydrates, mineral salts, vitamins, and various trace elements, with bile acids making up about 67% of the total composition. Bile acids are produced from excess cholesterol, secreted from the liver, absorbed into the small intestines, and returned to the liver with portal blood. While bile acid synthesis is critical for the removal of cholesterol from the body, bile acids are also needed for proper uptake of dietary lipids, fat soluble vitamins, and other nutrients into the small intestines. Under physiological conditions, newly synthesized bile acids are conjugated to glycine or taurine to form bile salts, and not much free bile acid is actually found in bile. Determining circulatory levels of bile acids can be used to identify or identify certain liver diseases. In addition, elevated serum bile levels have been observed in intrahepatic cholestasis of pregnancy cases. The Total Bile Acid Assay Kit is a simple colorimetric assay that measures the amount of total bile acid present in plasma, serum, tissue homogenates, or cell lysates in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, bile acid standards and unknown samples. Sample bile acid concentrations are determined by comparison with a known bile acid standard.
Product Categories/Family for Total Bile Acid assay kit
References
1. Reshetnyak V.I. (2013) World J. Gastro.. 19: 7341-7360.
2. Ambros-Rudoph C.M., Glatz M., Trauner M., Kerl H., and Mullegger R.R., (2007) Arch. Dermatol. 143: 757-762.
3. Angelin B., Bjorkhem I., and Einarsson K. (1978) J. Lipid Res. 19: 527-537.
4. Mashige F., Tanaka N., Maki A., Kami S., and Yamanaka M. (1981) Clin. Chem. 27: 1352-1356
5. Setchell K.D.R, Rodrigues C.M.P., Clerici C., Solinas A., Morelli A., Gartung C., and Boyer J. (1997) Gasteroenterolgy 112: 226-235
2. Ambros-Rudoph C.M., Glatz M., Trauner M., Kerl H., and Mullegger R.R., (2007) Arch. Dermatol. 143: 757-762.
3. Angelin B., Bjorkhem I., and Einarsson K. (1978) J. Lipid Res. 19: 527-537.
4. Mashige F., Tanaka N., Maki A., Kami S., and Yamanaka M. (1981) Clin. Chem. 27: 1352-1356
5. Setchell K.D.R, Rodrigues C.M.P., Clerici C., Solinas A., Morelli A., Gartung C., and Boyer J. (1997) Gasteroenterolgy 112: 226-235
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Product Notes
The Total Bile Acid (Catalog #AAA168476) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Total Bile Acid, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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