Rabbit GSTM1 Polyclonal Antibody | anti-GSTM1 antibody

GSTM1 Polyclonal Antibody

Cat. Num. AAA2554120

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antigen Affinity Purification
• Synonyms: GSTM1; Polyclonal Antibody; GSTM1 Polyclonal Antibody; GST class-mu 1; GST HB subunit 4; GST1; Gstm1; GSTM1a-1a; GTH4; GTM1; H-B; MU; anti-GSTM1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purification
• Concentration: 0.54 mg/mL (varies by lot)

• Applicable Applications for anti-GSTM1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB 1:500-1:6000; IHC 1:100-1:200; IF 1:100-1:200
• Immunogen: Recombinant Mouse Glutathione S-transferase Mu 1 protein
• Conjugation: Unconjugated
• Buffer: PBS with 0.05% Proclin300 and 50% glycerol, pH7.4.
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of Mouse liver, Mouse heart, NIH/3T3, Jurkat, Rat kidney, Rat heart and Mouse kidney using GSTM1 Polyclonal Antibody at dilution of 1:500)

Western Blot (WB)

(Western Blot analysis of Hela cells using GSTM1 Polyclonal Antibody at dilution of 1:500)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human liver cancer using GSTM1 Polyclonal Antibody at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human kidney using GSTM1 Polyclonal Antibody at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescence analysis of Hela cells using GSTM1 Polyclonal Antibody at dilution of 1:100)

Related Product Information for anti-GSTM1 antibody

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Null mutations of this class mu gene have been linked with an increase in a number of cancers, likely due to an increased susceptibility to environmental toxins and carcinogens. Multiple protein isoforms are encoded by transcript variants of this gene.

Product Categories/Family for anti-GSTM1 antibody

Tags & Cell Markers; Signal Transduction; Metabolism

NCBI and Uniprot Product Information

• UniProt Accession #: P10649
• Molecular Weight: 26 kDa
• Protein Family: Glutathione S-transferase

NCBI Description

This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.