Immunohistochemistry (IHC)
(Immunochemical staining of rat CD48 in rat thymus with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).)
Rabbit anti-Rat CD48 Polyclonal Antibody | anti-CD48 antibody
Anti-CD48 Antibody, Rabbit Polyclonal
Gene Names
Cd48; SLAMF2
Reactivity
Rat
Applications
Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
CD48; Polyclonal Antibody; Anti-CD48 Antibody; Rabbit Polyclonal; CD48/SLAMF2 Antibody; Rabbit PAb; Antigen Affinity Purified; CD48 molecule; anti-CD48 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rat CD48/SLAMF2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CD48 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
ELISA: 1:25,000-1:50,000
IHC-P: 1:10,000-1:50,000
IP: 0.2-1uL/mg of lysate
ELISA: 1:25,000-1:50,000
IHC-P: 1:10,000-1:50,000
IP: 0.2-1uL/mg of lysate
Immunogen
Recombinant Rat CD48/SLAMF2 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Rat CD48/SLAMF2. CD48/SLAMF2 specific IgG was purified by Rat CD48/SLAMF2 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of rat CD48 in rat thymus with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of rat CD48 in rat thymus with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).)
Immunoprecipitation (IP)
(rat CD48 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-rat CD48 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-rat CD48 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 35 kDa)
Immunoprecipitation (IP)
(rat CD48 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-rat CD48 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-rat CD48 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 35 kDa)
Western Blot (WB)
(Anti-CD48 rabbit polyclonal antibody at 1:500 dilution.Lane A: Jurkat Membrane LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:28 kDaObserved band size:48 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-CD48 rabbit polyclonal antibody at 1:500 dilution.Lane A: Jurkat Membrane LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:28 kDaObserved band size:48 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-CD48 antibody
Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.
Product Categories/Family for anti-CD48 antibody
References
Messmer B, et al. (2006) CD48 stimulation by 2B4 (CD244)-expressing targets activates human NK cells. J Immunol. 176(8): 4646-50Milstein O, et al. (2008) Nanoscale increases in CD2-CD48-mediated intermembrane spacing decrease adhesion and reorganize the immunological synapse. J Biol Chem. 283(49): 34414-22.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,680 Da
NCBI Official Full Name
CD48 antigen
NCBI Official Synonym Full Names
Cd48 molecule
NCBI Official Symbol
Cd48
NCBI Official Synonym Symbols
SLAMF2
NCBI Protein Information
CD48 antigen; BCM1 surface antigen; BLAST-1; MRC OX-45 surface antigen; SLAM family member 2; signaling lymphocytic activation molecule 2
UniProt Protein Name
CD48 antigen
Protein Family
UniProt Gene Name
Cd48
UniProt Synonym Gene Names
Bcm-1; SLAMF2
UniProt Entry Name
CD48_RAT
Similar Products
Product Notes
The CD48 cd48 (Catalog #AAA8112902) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD48 Antibody, Rabbit Polyclonal reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD48 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000 IHC-P: 1:10,000-1:50,000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the CD48 cd48 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD48, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
