Typical Testing Data/Standard Curve (for reference only)
Lipid Peroxidation (MDA) Assay Kit
Lipid Peroxidation (MDA) Assay kit
Synonyms
Lipid Peroxidation (MDA); Lipid Peroxidation (MDA) Assay kit; Lipid Peroxidation (MDA) assay kit
Application Notes
Lipid Peroxidation (MDA) Assay Kit provides a convenient tool for detection of the malondialdehyde (MDA) present in a variety of samples. MDA, together with 4-hydroxynonenal (4-HNE), is a natural bi-product of lipid peroxidation and its quantification is generally used as marker for lipid peroxidation. The MDA in the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct, which can be easily quantified colorimetrically at OD 532 nm. Meanwhile, the absorbance at 600 nm was measured, and MDA was calculated by the difference between the absorbance at 532 nm and 600 nm (To avoid the interference of saccharose).
Preparation and Storage
Store at -20 degree C immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Lipid Peroxidation (MDA) assay kit
Principle of the Assay: Lipid Peroxidation (MDA) Assay Kit provides a convenient tool for detection of the malondialdehyde (MDA) present in a variety of samples. MDA, together with 4-hydroxynonenal (4-HNE), is a natural bi-product of lipid peroxidation and its quantification is generally used as marker for lipid peroxidation. The MDA in the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct, which can be easily quantified colorimetrically at OD 532 nm. Meanwhile, the absorbance at 600 nm was measured and MDA was calculated by the difference between the absorbance at 532 nm and 600 nm (To avoid the interference of saccharose).
Background/Introduction: Oxidative stress has been implicated in the etiology of many chronic diseases, including cardiovascular disease. Lipid peroxidation is the degradation of lipids that occurs as a result of oxidative damage and is a useful marker for oxidative stress. Polyunsaturated lipids are susceptible to an oxidative attack, typically by reactive oxygen species, resulting in a well-defined chain reaction with the production of end products such as malondialdehyde (MDA). Lipid peroxidation may contribute to the pathology of many diseases including atherosclerosis, diabetes and Alzheimer's.
Background/Introduction: Oxidative stress has been implicated in the etiology of many chronic diseases, including cardiovascular disease. Lipid peroxidation is the degradation of lipids that occurs as a result of oxidative damage and is a useful marker for oxidative stress. Polyunsaturated lipids are susceptible to an oxidative attack, typically by reactive oxygen species, resulting in a well-defined chain reaction with the production of end products such as malondialdehyde (MDA). Lipid peroxidation may contribute to the pathology of many diseases including atherosclerosis, diabetes and Alzheimer's.
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Product Notes
The Lipid Peroxidation (MDA) (Catalog #AAA9718963) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Lipid Peroxidation (MDA) Assay Kit provides a convenient tool for detection of the malondialdehyde (MDA) present in a variety of samples. MDA, together with 4-hydroxynonenal (4-HNE), is a natural bi-product of lipid peroxidation and its quantification is generally used as marker for lipid peroxidation. The MDA in the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct, which can be easily quantified colorimetrically at OD 532 nm. Meanwhile, the absorbance at 600 nm was measured, and MDA was calculated by the difference between the absorbance at 532 nm and 600 nm (To avoid the interference of saccharose). . Researchers should empirically determine the suitability of the Lipid Peroxidation (MDA) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lipid Peroxidation (MDA), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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