Immunoblot
(The following figures demonstrate typical results. One should use the data below for reference only. This data should not be used to interpret actual results.Immunoblot of HNE-Modified BSA. Left: Ponceau S staining. Right: Immunoblot using Rabbit Anti-HNE Polyclonal Antibody at 1:1000 dilution, followed by HRP-conjugated secondary antibody. Numbers indicate ng/lane.)
Rabbit 4-Hydroxynonenal (HNE) Polyclonal Antibody
Rabbit Anti-4-Hydroxynonenal (HNE) Polyclonal Antibody
Applications
Immunoblot, Western Blot, ELISA
Purity
Affinity Purified
Synonyms
4-Hydroxynonenal (HNE); Polyclonal Antibody; Rabbit Anti-4-Hydroxynonenal (HNE) Polyclonal Antibody; anti-4-Hydroxynonenal (HNE) antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
HNE-modified proteins
Purity/Purification
Affinity Purified
Form/Format
PBS, pH 7.2, containing 0.5 mM EDTA, 0.02% NaN3, and 30% Glycerol
Concentration
0.5 mg/mL (varies by lot)
Applicable Applications for anti-4-Hydroxynonenal (HNE) antibody
Immunoblot (IB), ELISA (EIA)
Application Notes
Immunoblot (1:200 to 1:8000)
ELISA (1:200 to 1:8000)
ELISA (1:200 to 1:8000)
Immunogen
HNE-modified Blue Carrier
Preparation and Storage
-20 degree C. Shelf Life: 1 year from date of receipt under proper storage conditions; aliquot to avoid multiple freeze thaw cycles
Immunoblot
(The following figures demonstrate typical results. One should use the data below for reference only. This data should not be used to interpret actual results.Immunoblot of HNE-Modified BSA. Left: Ponceau S staining. Right: Immunoblot using Rabbit Anti-HNE Polyclonal Antibody at 1:1000 dilution, followed by HRP-conjugated secondary antibody. Numbers indicate ng/lane.)
Immunoblot
(The following figures demonstrate typical results. One should use the data below for reference only. This data should not be used to interpret actual results.Immunoblot of HNE-Modified BSA. Left: Ponceau S staining. Right: Immunoblot using Rabbit Anti-HNE Polyclonal Antibody at 1:1000 dilution, followed by HRP-conjugated secondary antibody. Numbers indicate ng/lane.)
Related Product Information for anti-4-Hydroxynonenal (HNE) antibody
Lipid peroxidation is a well-defined mechanism of cellular damage in animals and plants. Lipid peroxides are unstable indicators of oxidative stress in cells that decompose to form more complex and reactive compounds such as Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), natural bi-products of lipid peroxidation. Oxidative modification of lipids can be induced in vitro by a wide array of pro-oxidant agents and occurs in vivo during aging and in certain disease conditions. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage. These aldehydic secondary products of lipid peroxidation are generally accepted markers of oxidative stress.
Both MDA and HNE have been shown to be capable of binding to proteins and forming stable adducts, also termed advanced lipid peroxidation end products. These modifications of proteins by MDA or HNE can cause both structural and functional changes of oxidized proteins.
Both MDA and HNE have been shown to be capable of binding to proteins and forming stable adducts, also termed advanced lipid peroxidation end products. These modifications of proteins by MDA or HNE can cause both structural and functional changes of oxidized proteins.
Product Categories/Family for anti-4-Hydroxynonenal (HNE) antibody
Similar Products
Product Notes
The 4-Hydroxynonenal (HNE) (Catalog #AAA168532) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's 4-Hydroxynonenal (HNE) can be used in a range of immunoassay formats including, but not limited to, Immunoblot (IB), ELISA (EIA). Immunoblot (1:200 to 1:8000) ELISA (1:200 to 1:8000). Researchers should empirically determine the suitability of the 4-Hydroxynonenal (HNE) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "4-Hydroxynonenal (HNE), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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