Testing Data
OxiSelect 8-iso-Prostaglandin F2a ELISA Kit
OxiSelect 8-iso-Prostaglandin F2a ELISA Kit
Synonyms
OxiSelect 8-iso-Prostaglandin F2a; OxiSelect 8-iso-Prostaglandin F2a ELISA Kit; OxiSelect 8-iso-Prostaglandin F2a elisa kit
Samples
Plasma, urine, serum, or tissue extracts
Preparation and Storage
Upon receipt, store the Anti-8-iso-PGF2alpha Antibody, 8-iso-PGF2alpha-HRP Conjugate, and 8-iso-PGF2alpha Standard at -20 degree C. Make aliquots as necessary to avoid freeze/thaw cycles. Store all other kit components at 4 degree C. Any partial or unused components should return to their proper storage temperatures.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for OxiSelect 8-iso-Prostaglandin F2a elisa kit
Principle of the assay: 8-iso-PGF2alpha kit is a competitive enzyme-linked immunoassay (ELISA) for determining levels of 8-iso-PGF2alpha in a variety of biological samples such as plasma, urine, serum, or tissue extracts. An antibody to 8-iso-PGF2alpha is incubated in pre-coated microtiter plate wells. Upon washing, 8-iso-PGF2alpha standards or treated samples are mixed with an 8-iso-PGF2alpha-HRP conjugate and added simultaneously to the wells. The unconjugated, or free 8-iso-PGF2alpha and 8-iso-PGF2alpha-HRP conjugate compete for binding to the antibody bound to the plate. After this brief incubation and wash, a substrate to the HRP is added. The HRP activity results in color development that is directly proportional to the amount of 8-iso-PGF2alpha conjugate bound to the plate and inversely proportional to the amount of free 8-iso-PGF2alpha in the samples or standards. The 8-iso-PGF2alpha content in an unknown sample is determined by comparing with the known predetermined standard curve. Please read the complete kit insert prior to performing the assay.
Background: Lipid peroxidation is a well-defined mechanism of cellular damage in animals and plants. Lipid peroxides are unstable indicators of oxidative stress in cells that decompose to form more complex and reactive compounds such as isoprostanes. The isoprostanes are a type of eicosanoids produced non-enzymatically through the oxygen radical induced peroxidation of tissue phospholipids and lipoproteins. Isoprostanes are prostaglandin-like compounds that appear in normal plasma and urine samples, but are elevated by oxidative stress in tissue, plasma, and urine. 8-iso-Prostaglandin F2alpha (also known as 8-epi-PGF2alpha, 8-isoprostane, or 15-isoprostane F2t), is an isoprostane that has been shown to be useful for the assessment of oxidative stress in vivo. It is produced in membrane phospholipids from non-cyclooxygenase and cyclooxygenase peroxidation pathways derived from arachidonic acid. 8-iso-Prostaglandin F2alpha (8-iso-PGF2alpha) is a potent vasoconstrictor, a mutagen in 3T3 cells as well as vascular smooth muscle cells, and also a possible pathophysiological mediator that can alter membrane integrity. It has been implicated in atherogenesis and elevated levels are associated with hepatorenal syndrome, rheumatoid arthritis, carcinogenesis, as well as atherosclerosis. 8-iso-PGF2alpha circulates in the plasma and is excreted in the urine. 8-iso PGF2alpha circulates as an esterified LDL Phospholipid and as a free acid. Normal human plasma and urine 8-iso PGF2alpha is about 40-100 pg/mL and about 190 pg/mg of creatinine respectively. Methods for determining total 8-iso PGF2alpha usually require alkaline hydrolysis of 8-iso PGF2alpha esters from tissues followed by extractions, phase separations and thin layer chromatography. The OxiSelect 8-iso-Prostaglandin F2alpha ELISA Kit is an enzyme immunoassay developed for rapid detection and quantification of 8-iso-PGF2alpha. The quantity of 8-iso-PGF2alpha in samples is determined by comparing its absorbance with that of a known 8-iso-PGF2alpha standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including the standard curve and unknown samples.
Product Categories/Family for OxiSelect 8-iso-Prostaglandin F2a elisa kit
References
1. Banerjee, M., Kang, K.H., Morrow, J.D., et al. (1992) Am. J. Physiol. 263: H660-H663.
2. Morrow, J.D., Hill, K.E., Burk, R.F., et al. (1990) Proc. Natl. Acad. Sci. USA. 87: 9383-9387.
3. Morrow, J.D., Harris, T.M., Roberts, L.J. (1990) Anal. Biochem. 184: 1-10.
4. Vacchiano, C.A., and Tempel, G.E. (1994) J. Appl. Physiol. 77: 2912-2917.
5. Wang, Z., Ciabattoni, G., Cre'minon, C., et al. (1995) Pharmacol. Exp. Ther. 275: 94-100.
1. Paneni, F. et al. (2015). Adverse epigenetic signatures by histone methyltransferase set7 contribute to vascular dysfunction in patients with type 2 diabetes mellitus. Circ Cardiovasc Genet. 8:150-158. 2. Dallatu, M. K. et al. (2015). The role of hypoxia-inducible factor/prolyl hydroxylation pathway in deoxycorticosterone acetate/salt hypertension in the rat. J Hypertens. doi:10.4172/2167-1095.1000184. 3. Pereira, S. et al. (2015). Effect of N-acetyl-L-cysteine on insulin resistance caused by prolonged FFA elevation. J Endocrinol. doi: 10.1530/JOE-14-0676. 4. Zabala, V. et al. (2015). Potential contributions of the tobacco nicotine-derived nitrosamine ketone (NNK) in the pathogenesis of steatohepatitis in a chronic plus binge rat model of alcoholic liver disease. Alcohol Alcohol. doi: http://dx.doi.org/10.1093/alcalc/agu083. 5. Macedo, R. C. S. et al. (2015). Effects of chronic resveratrol supplementation in military firefighters undergo a physical fitness test-a placebo-controlled, double blind study. Chem Biol Interact. 227:89-95.6. Dugas, T.R. et al. (2014). Hydrogen sulfide cytoprotective signaling is endothelial nitric oxide synthase-nitric oxide dependent. PNAS 111:3182-3187.7. Karakus, E. et al. (2013). Agomelatine: an antidepressant with new potent hepatoprotective effects on paracetamol-induced liver damage in rats. Human and Experimental Toxicology. 10.01177/0960327112472994.8. Mollo, R.et al. (2012). Effect of alpha-lipoic acid on platelet reactivity in type 1 diabetic patients. Diabetes Care 35:196-197.9. Thompson, C.M. et al. (2012). Comparison of the effects of hexavalent chromium in the alimentary canal of F344 rats and B6C3F1 mice following exposure in drinking water: implications for carcinogenic modes of action. Toxicol. Sci. 125:79-90.10. Ungvari, Z. et al. (2011). Age-associated vascular oxidative stress, Nrf2 dysfunction, and NF-kappaB activation in the nonhuman primate Macaca mulatta. J. Gerontol A Biol Sci Med Sci. 10.1093/gerona/glr092.
2. Morrow, J.D., Hill, K.E., Burk, R.F., et al. (1990) Proc. Natl. Acad. Sci. USA. 87: 9383-9387.
3. Morrow, J.D., Harris, T.M., Roberts, L.J. (1990) Anal. Biochem. 184: 1-10.
4. Vacchiano, C.A., and Tempel, G.E. (1994) J. Appl. Physiol. 77: 2912-2917.
5. Wang, Z., Ciabattoni, G., Cre'minon, C., et al. (1995) Pharmacol. Exp. Ther. 275: 94-100.
1. Paneni, F. et al. (2015). Adverse epigenetic signatures by histone methyltransferase set7 contribute to vascular dysfunction in patients with type 2 diabetes mellitus. Circ Cardiovasc Genet. 8:150-158. 2. Dallatu, M. K. et al. (2015). The role of hypoxia-inducible factor/prolyl hydroxylation pathway in deoxycorticosterone acetate/salt hypertension in the rat. J Hypertens. doi:10.4172/2167-1095.1000184. 3. Pereira, S. et al. (2015). Effect of N-acetyl-L-cysteine on insulin resistance caused by prolonged FFA elevation. J Endocrinol. doi: 10.1530/JOE-14-0676. 4. Zabala, V. et al. (2015). Potential contributions of the tobacco nicotine-derived nitrosamine ketone (NNK) in the pathogenesis of steatohepatitis in a chronic plus binge rat model of alcoholic liver disease. Alcohol Alcohol. doi: http://dx.doi.org/10.1093/alcalc/agu083. 5. Macedo, R. C. S. et al. (2015). Effects of chronic resveratrol supplementation in military firefighters undergo a physical fitness test-a placebo-controlled, double blind study. Chem Biol Interact. 227:89-95.6. Dugas, T.R. et al. (2014). Hydrogen sulfide cytoprotective signaling is endothelial nitric oxide synthase-nitric oxide dependent. PNAS 111:3182-3187.7. Karakus, E. et al. (2013). Agomelatine: an antidepressant with new potent hepatoprotective effects on paracetamol-induced liver damage in rats. Human and Experimental Toxicology. 10.01177/0960327112472994.8. Mollo, R.et al. (2012). Effect of alpha-lipoic acid on platelet reactivity in type 1 diabetic patients. Diabetes Care 35:196-197.9. Thompson, C.M. et al. (2012). Comparison of the effects of hexavalent chromium in the alimentary canal of F344 rats and B6C3F1 mice following exposure in drinking water: implications for carcinogenic modes of action. Toxicol. Sci. 125:79-90.10. Ungvari, Z. et al. (2011). Age-associated vascular oxidative stress, Nrf2 dysfunction, and NF-kappaB activation in the nonhuman primate Macaca mulatta. J. Gerontol A Biol Sci Med Sci. 10.1093/gerona/glr092.
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Product Notes
The OxiSelect 8-iso-Prostaglandin F2a (Catalog #AAA168454) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "OxiSelect 8-iso-Prostaglandin F2a, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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