Rabbit anti-Human, Mouse RBM4 Polyclonal Antibody | anti-RBM4 antibody

RBM4 Polyclonal Antibody

Cat. Num. AAA2563076

• Gene Names: RBM4; LARK; RBM4A; ZCRB3A; ZCCHC21
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Antigen Affinity Purification
• Synonyms: RBM4; Polyclonal Antibody; RBM4 Polyclonal Antibody; dkfzp547k0918; HLark; Lark; Lark homolog; Lark homologue; Mgc75138; Rbm4 lark; RNA binding motif 4; RNA binding motif 4a; RNA-binding motif protein 4; RNA-binding motif protein 4a; RNA-binding protein 4; anti-RBM4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purification
• Form/Format: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
• Concentration: 0.66mg/mL (varies by lot)
• Sequence Length: 364

• Applicable Applications for anti-RBM4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10000
• Immunogen: Fusion protein of human RBM4
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of Hela and MCF7 cell lysates using RBM4 Polyclonal Antibody at dilution of 1:750)

Related Product Information for anti-RBM4 antibody

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis.

Product Categories/Family for anti-RBM4 antibody

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 62511089
• NCBI GeneID: 5936
• UniProt Accession #: Q9BWF3
• Molecular Weight: 40 kDa
• NCBI Official Full Name: RNA-binding protein 4
• NCBI Official Synonym Full Names: RNA binding motif protein 4
• NCBI Official Symbol: RBM4
• NCBI Official Synonym Symbols: LARK; RBM4A; ZCRB3A; ZCCHC21
• NCBI Protein Information: RNA-binding protein 4
• UniProt Protein Name: RNA-binding protein 4
• Protein Family: RNA-binding protein
• UniProt Gene Name: RBM4
• UniProt Synonym Gene Names: RBM4A; hLark
• UniProt Entry Name: RBM4_HUMAN

Uniprot Description

RBM4: RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs. Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3'-UTR of the PER1 mRNA. Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3'UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation. Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress. Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions. Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of EIF2C2- containing miRNPs with their cognate target mRNAs. Associates with miRNAs during muscle cell differentiation. Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Nucleolus; RNA splicing; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; stress granule; cytoplasm; nucleolus; nuclear speck; nucleus

Molecular Function: mRNA binding; protein binding; miRNA binding; mRNA 3'-UTR binding; zinc ion binding; RNA binding; nucleotide binding

Biological Process: negative regulation of translational initiation; RNA splicing; response to arsenic; stress-activated MAPK cascade; miRNA-mediated gene silencing, negative regulation of translation; entrainment of circadian clock by photoperiod; RNA processing; negative regulation of translation in response to stress; regulation of nucleocytoplasmic transport; negative regulation of translation; positive regulation of muscle cell differentiation; cell differentiation; mRNA processing; regulation of alternative nuclear mRNA splicing, via spliceosome