Rabbit anti-Human KSR Polyclonal Antibody | anti-KSR1 antibody

KSR, NT (Kinase Suppressor of Ras 1, KSR1, RSU2)

Cat. Num. AAA630185

• Gene Names: KSR1; KSR; RSU2
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: KSR; Polyclonal Antibody; NT (Kinase Suppressor of Ras 1; KSR1; RSU2); Anti -KSR; anti-KSR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KSR1.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-KSR1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500
• Immunogen: Synthetic peptide selected from the N-terminal region of human KSR1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-KSR1 Pab in T47D cell line lysate. KSR1(arrow) was detected using the purified Pab.)

Related Product Information for anti-KSR1 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Product Categories/Family for anti-KSR1 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 75677325
• NCBI GeneID: 8844
• NCBI Accession #: NP_055053.1
• NCBI GenBank Nucleotide #: NM_014238.1
• UniProt Accession #: Q8IVT5; Q13476
• Molecular Weight: 102,032 Da
• NCBI Official Full Name: kinase suppressor of Ras 1
• NCBI Official Synonym Full Names: kinase suppressor of ras 1
• NCBI Official Symbol: KSR1
• NCBI Official Synonym Symbols: KSR; RSU2
• NCBI Protein Information: kinase suppressor of Ras 1
• UniProt Protein Name: Kinase suppressor of Ras 1
• Protein Family: Kinase suppressor of Ras
• UniProt Gene Name: KSR1
• UniProt Synonym Gene Names: KSR
• UniProt Entry Name: KSR1_HUMAN

Uniprot Description

KSR: a serine/threonine kinase of the Raf family with no demonstrated kinase activity. A location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. Interacts with RAF and MAPK/ERK, in a Ras-dependent manner. The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization. In unstimulated cells, the phosphorylated form is bound to a 14-3-3 protein and sequestered in the cytoplasm. Translocates from the cytoplasm to the cell periphery following TNF treatment. When Bad is available, ceramide signaling through the Ras/Raf cascade can lead to apoptosis. Three alternatively-spliced isoforms have been described.

Protein type: Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; RAF family

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum

Molecular Function: protein serine/threonine kinase activity; MAP-kinase scaffold activity; protein binding; mitogen-activated protein kinase kinase binding; metal ion binding; ATP binding; protein kinase activity

Biological Process: positive regulation of MAPKKK cascade; Ras protein signal transduction; protein amino acid phosphorylation