Typical Testing Data/Standard Curve (for reference only)
Phospholipid Assay Kit
Phospholipid Assay Kit
Synonyms
Phospholipid; Phospholipid Assay Kit; Phospholipid assay kit
Preparation and Storage
Store the Hydrolyzing Enzyme at -80 degree C. Store the Phospholipid Standard, Fluorescence Probe, HRP, and Oxidoreductase at -20 degree C or lower. Store the remaining kit components at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Phospholipid assay kit
Principle of the Assay: The Phospholipid Assay Kit measures phospholipids through a series of enzyme driven reactions. First, a hydrolyzing enzyme reacts with phospholipids, such as lecithins and sphingomyelins, breaking the phosphodiester bond within their structure. Next, an oxidoreductase reacts to generate a peroxide, which is subsequently detected with a highly specific fluorescence probe. Samples and standards are incubated for 30 minutes and then read with a standard 96-well fluorometric plate reader (Ex. 530-570 nm/Em. 590-600 nm). Sample phospholipid levels are determined by comparison with known phospholipid standards.!!Background/Introduction: Phospholipids are important structural lipids that are the major component of cell membranes and lipid bilayers. They contain a hydrophilic head and a hydrophobic tail which give the molecules their unique amphiphilic characteristics. Most phospholipids contain one diglyceride, a phosphate group, and one choline group (Figure 1). Sphingomyelin, lysophosphatidylcholine (lysolecithin), and phosphatidylcholine (lecithin) are predominantly choline-based phospholipids which account for 95% of phospholipids in human serum and plasma. Phosphatidylcholine is the foremost membrane phospholipid in eukaryotic cell membranes and is present in every cell in the body. Determining circulatory levels of phospholipids and lipoproteins is critical to the identification of lipid transport disorders and may be used as markers for heart, liver, or lung diseases. Phospholipid biosynthesis and metabolism is important to membrane structure maintenance and function. Many exert considerable influence on lipid homeostasis. They serve as a pool for many lipid messengers and are a source for bioactive lipids such as phosphatidic acid, diacylglycerol, lysophosphatidylcholine, and others. Phospholipids supply choline in the body, which itself and its derivative compounds form cell signaling molecules such as acetylcholine, platelet activating factor and sphingophosphorylcholine. Energy production and storage, prostaglandin production, blood clotting, cholesterol solubility, fat and bile emulsification, and antioxidant activity are pathways involving phospholipid activity. Due to their decreased toxicity, improved bioavailability and excellent permeability, phospholipids are used for liposomal, ethosomal and other nanoparticle formulations used in drug delivery systems. The Phospholipid Assay Kit is a simple fluorometric assay that measures the amount of choline-based phospholipids present within plasma, serum, tissue homogenates, or cell suspensions in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, phospholipid standards and samples. The kit's detection sensitivity limit is approximately 150 nM.
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Product Notes
The Phospholipid (Catalog #AAA169549) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Phospholipid, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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