Typical Testing Data/Standard Curve (for reference only)
Bilirubin Assay Kit
Bilirubin (Total and Direct) Colorimetric Assay Kit
Synonyms
Bilirubin; Bilirubin (Total and Direct) Colorimetric Assay Kit; Bilirubin assay kit
Application Notes
Measurement of Bilirubin in Serum samples
Samples
Biological fluids: Serum
Detection Method
Absorbance (600 nm for Total Bilirubin and 550 nm for Direct Bilirubin)
Features & Benefits
Simple protocol, robust and reliable data
This assay allows detection of both Total and Direct or conjugated Bilirubin in serum samples.
This assay allows detection of both Total and Direct or conjugated Bilirubin in serum samples.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Bilirubin assay kit
Product Information: Total and Direct Bilirubin Assay Kit: Colorimetric Assay to Measure Bilirubin (Total or Direct) in serum samples. Simple & Convenient. 100 Assays.
Background: Bilirubin, a degradation product of heme catabolism, is a non-polar molecule. There are two forms of bilirubin: water-soluble (conjugated or direct) and water-insoluble (unconjugated or indirect) bilirubin. Bilirubin is produced in the endoplasmic reticulum as unconjugated bilirubin, which binds to albumin in plasma and forms albumin-bilirubin complex. This complex is transported to the liver, where it is conjugated with glucuronic acid and forms conjugated bilirubin. Bilirubin has potent antioxidant, anti-inflammatory and autoimmune properties. Bilirubin concentration in human body depends on gender, drug intake, age, etc. Low serum bilirubin is directly correlated with pathological conditions including diabetes mellitus, metabolic syndrome, and cardiovascular diseases. However, high bilirubin indicates hemolysis, jaundice, Gilbert's syndrome, hepatitis, drug toxicity, and possible blockage of bile ducts. MyBioSource's Bilirubin Assay Kit utilizes the Jendrassik-Grof principle to detect bilirubin. Total bilirubin (unconjugated + conjugated) concentration is determined in the presence of a catalyst, where bilirubin reacts with a diazo- salt to form azobilirubin, which absorbs at 600 nm. Direct bilirubin (conjugated) is determined in the absence of catalyst (550 nm)..
Summary: Detection method- Absorbance (600 nm for Total Bilirubin and 550 nm for Direct Bilirubin)
Application- Measurement of Bilirubin in Serum samples
Background: Bilirubin, a degradation product of heme catabolism, is a non-polar molecule. There are two forms of bilirubin: water-soluble (conjugated or direct) and water-insoluble (unconjugated or indirect) bilirubin. Bilirubin is produced in the endoplasmic reticulum as unconjugated bilirubin, which binds to albumin in plasma and forms albumin-bilirubin complex. This complex is transported to the liver, where it is conjugated with glucuronic acid and forms conjugated bilirubin. Bilirubin has potent antioxidant, anti-inflammatory and autoimmune properties. Bilirubin concentration in human body depends on gender, drug intake, age, etc. Low serum bilirubin is directly correlated with pathological conditions including diabetes mellitus, metabolic syndrome, and cardiovascular diseases. However, high bilirubin indicates hemolysis, jaundice, Gilbert's syndrome, hepatitis, drug toxicity, and possible blockage of bile ducts. MyBioSource's Bilirubin Assay Kit utilizes the Jendrassik-Grof principle to detect bilirubin. Total bilirubin (unconjugated + conjugated) concentration is determined in the presence of a catalyst, where bilirubin reacts with a diazo- salt to form azobilirubin, which absorbs at 600 nm. Direct bilirubin (conjugated) is determined in the absence of catalyst (550 nm)..
Summary: Detection method- Absorbance (600 nm for Total Bilirubin and 550 nm for Direct Bilirubin)
Application- Measurement of Bilirubin in Serum samples
Product Categories/Family for Bilirubin assay kit
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Product Notes
The Bilirubin (Catalog #AAA841556) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of Bilirubin in Serum samples. Researchers should empirically determine the suitability of the Bilirubin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bilirubin, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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