Testing Data
(Kinase Assay: 4.96–62.5ng of this lot of enzyme phosphorylated 0.33mg/ml active p38δ/SAPK4. Test results are shown to the right. Assay background was subtracted from the actual counts to yield the results shown below.)
SAPK4, p38 delta, active Active Protein
SAPK4, p38 delta, active, Recombinant, Human (Stress-activated Protein Kinase 4, Mitogen-activated Protein Kinase 13, MAP Kinase 13, MAPK 13, MAPK13, PRKM13, Mitogen-activated Protein Kinase p38 delta, MAP Kinase p38 delta, p38delta)
Purity
Affinity PurifiedPurified to 50% by affinity chromatography on glutathione Sepharose beads after activation with SKK3/MEK6.
Synonyms
SAPK4; p38 delta; active; Recombinant; Human (Stress-activated Protein Kinase 4; Mitogen-activated Protein Kinase 13; MAP Kinase 13; MAPK 13; MAPK13; PRKM13; Mitogen-activated Protein Kinase p38 delta; MAP Kinase p38 delta; p38delta); active active protein
Purity/Purification
Affinity Purified
Purified to 50% by affinity chromatography on glutathione Sepharose beads after activation with SKK3/MEK6.
Purified to 50% by affinity chromatography on glutathione Sepharose beads after activation with SKK3/MEK6.
Form/Format
Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 0.15M NaCl, 0.1mM EGTA, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol, and 50% glycerol.
Specific Activity
~100U/mg where 1unit pf p38 delta/SAPK4 activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein/minute at 30 degree C with a final ATP of 100uM.
Preparation and Storage
Stable for 6 months at -20 degree C from date of shipment.
Testing Data
(Kinase Assay: 4.96–62.5ng of this lot of enzyme phosphorylated 0.33mg/ml active p38δ/SAPK4. Test results are shown to the right. Assay background was subtracted from the actual counts to yield the results shown below.)
Testing Data
(Kinase Assay: 4.96–62.5ng of this lot of enzyme phosphorylated 0.33mg/ml active p38δ/SAPK4. Test results are shown to the right. Assay background was subtracted from the actual counts to yield the results shown below.)
SDS-Page
(SDS-PAGE and Coomassie Stain: Purity was assessed by|SDS-PAGE and Coomassie blue staining using 3ug of S0096-52.)
SDS-Page
(SDS-PAGE and Coomassie Stain: Purity was assessed by|SDS-PAGE and Coomassie blue staining using 3ug of S0096-52.)
Related Product Information for SAPK4, p38 delta, active active protein
Recombinant GST fusion protein fused to full length human SAPK4, expressed in E. coli.
Human stress-activated protein kinase-4 (SAPK4) is a novel MAP Kinase family member whose amino acid sequence is 60% identical to that of the other three SAP Kinases which contain a TGY motif in their activation domain. Recent results suggest that cellular functions attributed to SAPK1 and/or SAPK2 may be mediated by either SAPK3 or SAPK4.
Human stress-activated protein kinase-4 (SAPK4) is a novel MAP Kinase family member whose amino acid sequence is 60% identical to that of the other three SAP Kinases which contain a TGY motif in their activation domain. Recent results suggest that cellular functions attributed to SAPK1 and/or SAPK2 may be mediated by either SAPK3 or SAPK4.
Product Categories/Family for SAPK4, p38 delta, active active protein
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Product Notes
The SAPK4, p38 delta, active (Catalog #AAA638322) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SAPK4, p38 delta, active, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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