Rabbit anti-Human LIN28A Polyclonal Antibody | anti-LIN28A antibody

Anti-LIN28A Antibody, Rabbit Polyclonal

Cat. Num. AAA8100367

• Gene Names: LIN28A; CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: LIN28A; Polyclonal Antibody; Anti-LIN28A Antibody; Rabbit Polyclonal; Lin28A Antibody; Rabbit PAb; Antigen Affinity Purified; lin-28 homolog A (C. elegans); Anti-CSDD1 Antibody; Anti-LIN-28 Antibody; Anti-lin-28A Antibody; Anti-LIN28 Antibody; Anti-ZCCHC1 Antibody; anti-LIN28A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human LIN28A
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-LIN28A antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:100-1:500
• Immunogen: A synthetic peptide corresponding to the center region of the human Lin28A
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with A synthetic peptide corresponding to the center region of the human Lin28A, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human LIN28A in human testis with rabbit polyclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human LIN28A in human glioma with rabbit polyclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-LIN28A antibody

LIN28A (Lin-28 Homolog A) is a Protein Coding gene. LIN28A, a highly-conserved RNA binding protein expressed during embryogenesis, plays roles in development, pluripotency, metabolism, as well as tissue repair in adults. The encoded protein functions through direct interaction with target mRNAs and by disrupting the maturation of certain miRNAs involved in embryonic development. LIN28 plays a critical role in the developmental transition, glucose metabolism, and tumorigenesis. At the molecular level, LIN28 is known to repress the maturation of let-7 microRNAs and enhance the translation of certain mRNAs. Aberrant expression of this gene is associated with cancer progression in multiple tissues. LIN28A is an important factor in tumor cells and ESCs, it is an inhibitor of the intracellular endoplasmic reticulum (ER)related protein translation in ESCs.

NCBI and Uniprot Product Information

• NCBI GI #: 13375938
• NCBI GeneID: 79727
• NCBI Accession #: NP_078950.1
• NCBI GenBank Nucleotide #: NM_024674.4
• UniProt Accession #: Q9H9Z2
• Molecular Weight: 22,743 Da
• NCBI Official Full Name: protein lin-28 homolog A
• NCBI Official Synonym Full Names: lin-28 homolog A (C. elegans)
• NCBI Official Symbol: LIN28A
• NCBI Official Synonym Symbols: CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A
• NCBI Protein Information: protein lin-28 homolog A; RNA-binding protein LIN-28; zinc finger CCHC domain-containing protein 1; zinc finger, CCHC domain containing 1
• UniProt Protein Name: Protein lin-28 homolog A
• Protein Family: Protein
• UniProt Gene Name: LIN28A
• UniProt Synonym Gene Names: CSDD1; LIN28; ZCCHC1; Lin-28A
• UniProt Entry Name: LN28A_HUMAN

Uniprot Description

LIN28A: Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let- 7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre- let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Monomer. During skeletal muscle differentiation, associated with translation initiation complexes in the polysomal compartment. Directly interacts with EIF3S2. Interaction with NCL is RNA-dependent. Interacts with ZCCHC11/TUT4. Can be negatively regulated by the interaction of microRNAs miR-125a and miR-125b with at least two miRNA responsive elements (miREs) in the 3'-UTR of this gene. These interactions may reduce both translation efficiency and mRNA abundance. Negatively regulated by retinoic acid. Expressed in embryonic stem cells (ES cells), placenta and testis. Belongs to the lin-28 family.

Protein type: Translation; RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: stress granule; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: mRNA binding; protein binding; miRNA binding; DNA binding; zinc ion binding; RNA binding; translation initiation factor binding

Biological Process: positive regulation of translation; regulation of transcription, DNA-dependent; pre-microRNA processing; somatic stem cell maintenance; stem cell maintenance; positive regulation of neuron differentiation; RNA 3'-end processing; germ cell development; negative regulation of glial cell differentiation