Testing Data
(Sample enzyme activity data)
p70 S6 Kinase Active Protein | prkacb active protein
p70 S6 Kinase (T412E), active, Recombinant (p70 S6K)
Gene Names
prkacb; pkacb; XPKAbeta
Purity
Highly Purified~ 90%. Purified by Ni-NTA agarose, activated with PDK1 and re-purified using Heparin Sepharose Chromatography. Purity is verified by SDS-PAGE.
Synonyms
p70 S6 Kinase; p70 S6 Kinase (T412E); active; Recombinant (p70 S6K); prkacb active protein
Purity/Purification
Highly Purified
~ 90%. Purified by Ni-NTA agarose, activated with PDK1 and re-purified using Heparin Sepharose Chromatography. Purity is verified by SDS-PAGE.
~ 90%. Purified by Ni-NTA agarose, activated with PDK1 and re-purified using Heparin Sepharose Chromatography. Purity is verified by SDS-PAGE.
Form/Format
Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 270mM sucrose, 0.15M sodium chloride, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij-35.
Specific Activity
~200U/mg
Unit Definition
One unit of p70 S6 kinase activity is defined as 1nmol phosphate incorporated into 100uM S6 Kinase/Rsk-2 Substrate Peptide 2 (KKRNRTLTK) per minute at 30 degree C with a final ATP concentration of 100uM.
Preparation and Storage
May be stored at -20 degree C for short-term only. For long-term storage, store at -70 degree C. Aliquots are stable for at least 6 months at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Sample enzyme activity data)
Testing Data
(Sample enzyme activity data)
Related Product Information for prkacb active protein
A truncated, N-terminus His-tagged fusion protein corresponding to aa 1-421 of human p70 S6 Kinase (containing a T412E mutation and a D18H mutation) expressed in Sf21 cells.
Product Categories/Family for prkacb active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48.7kD
NCBI Official Full Name
protein kinase, cAMP-dependent, catalytic, beta
NCBI Official Synonym Full Names
protein kinase, cAMP-dependent, catalytic, beta
NCBI Official Symbol
prkacb
NCBI Official Synonym Symbols
pkacb; XPKAbeta
NCBI Protein Information
protein kinase, cAMP-dependent, catalytic, beta; p70 S6 kinase
Protein Family
Similar Products
Product Notes
The prkacb (Catalog #AAA637756) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "p70 S6 Kinase, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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